Ghanbarei Solaleh, Sattarahmady Naghmeh, Zarghampoor Farzaneh, Azarpira Negar, Hossein-Aghdaie Mahdokht
Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran.
Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Biotechnol Lett. 2021 Aug;43(8):1659-1673. doi: 10.1007/s10529-021-03134-w. Epub 2021 May 2.
An attractive cell source for stem cell-based therapy are WJ-MSCs. Hence, tracking WJ-MSCs using non-invasive imaging procedures (such as MRI) and contrast agents (Zn0.5Ni0.5Fe2O4, NFNPs) are required to evaluate cell distribution, migration, and differentiation.
Results showed that the bare and dextrin-coated NFNPs were internalized inside the WJ-MSCs and had no effect on the cell viability, proliferation, apoptosis, karyotyping, and morphology of WJ-MSCs up to 125 µg/mL. Besides, treated WJ-MSCs were differentiated into osteo/adipocyte-like cells. The expression of RUNX 2, SPP 1 (P < 0.05), and OCN (P > 0.05) genes in the WJ-MSCs treated with dextrin-coated NFNPs was higher than the untreated WJ-MSCs; and the expression of CFD, LPL, and PPAR-γ genes was reduced in WJ-MSCs treated with both NFNPs in comparison with the untreated WJ-MSCs (P > 0.05).
Overall, results showed that dextrin-coated NFNPs had no adverse effect on the cellular characteristics, proliferation, and differentiation of WJ-MSCs, and suggesting their potential clinical efficacy.
基于干细胞的治疗中，一种有吸引力的细胞来源是羊膜间充质干细胞（WJ-MSCs）。因此，需要使用非侵入性成像程序（如MRI）和造影剂（Zn0.5Ni0.5Fe2O4，NFNPs）来追踪WJ-MSCs，以评估细胞分布、迁移和分化情况。
结果表明，裸露的和糊精包被的NFNPs被WJ-MSCs内化，在浓度高达125µg/mL时，对WJ-MSCs的细胞活力、增殖、凋亡、核型分析和形态均无影响。此外，经处理的WJ-MSCs可分化为骨/脂肪细胞样细胞。用糊精包被的NFNPs处理的WJ-MSCs中RUNX 2、SPP 1（P<0.05）和OCN（P>0.05）基因的表达高于未处理的WJ-MSCs；与未处理的WJ-MSCs相比，两种NFNPs处理的WJ-MSCs中CFD、LPL和PPAR-γ基因的表达均降低（P>0.05）。
总体而言，结果表明糊精包被的NFNPs对WJ-MSCs的细胞特性、增殖和分化没有不良影响，并提示了它们潜在的临床疗效。
