College of Food Science, South China Agricultural University, Guangzhou 510642, China.
College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Food Res Int. 2023 Oct;172:112562. doi: 10.1016/j.foodres.2023.112562. Epub 2023 Feb 6.
Daylily (Hemerocallis citrina Baroni, HC) is an edible plant and is traditionally considered with potential to improve sleep. Herein, based on the Drosophila activity monitoring, metabolome, targeted screening and transcriptome, the material basis and mechanism of HC on sleep-improvement was investigated. The results showed that the aqueous extracts of HC (HAE) as well as the ethanol extracts (HEE) all prolonged the total sleep time of insomnia fruit flies, especially HEE-60 and HEE-95 exhibited more significant effects. In addition, 539 of 728 found metabolites were screened as potential sleep-improved metabolites, and quercetin, linoleic acid, phenethyl caffeate, L-methionine and γ-aminobutyric acid were considered as core active metabolites. Meanwhile, 368 differentially expressed genes (DEGs) were revealed by transcriptomics analysis, and the neuroactive ligand-receptor interaction was deduced as the main pathway by KEGG pathway enrichment. Furthermore, nine DEGs located in this pathway, namely betaTry, deltaTry, gammaTry, epsilonTry, etaTry, iotaTry, lambdaTry, kappaTry and CG30031 were proven being up-regulated. All these results contribute to the development of HC-related functional foods.
萱草（Hemerocallis citrina Baroni，HC）是一种食用植物，传统上被认为具有改善睡眠的潜力。本研究基于果蝇活动监测、代谢组学、靶向筛选和转录组学，探讨了 HC 改善睡眠的物质基础和作用机制。结果表明，萱草的水提物（HAE）和醇提物（HEE）均能延长失眠果蝇的总睡眠时间，尤其是 HEE-60 和 HEE-95 的效果更为显著。此外，共筛选出 728 种代谢物中的 539 种作为潜在的改善睡眠的代谢物，其中槲皮素、亚油酸、咖啡酸苯乙酯、L-蛋氨酸和γ-氨基丁酸被认为是核心活性代谢物。同时，转录组学分析揭示了 368 个差异表达基因（DEGs），KEGG 通路富集分析推断神经活性配体-受体相互作用是主要通路。进一步验证了该通路中 9 个位于该通路中的 DEGs，即 betaTry、deltaTry、gammaTry、epsilonTry、etaTry、iotaTry、lambdaTry、kappaTry 和 CG30031，它们均被证明上调。这些结果为开发与 HC 相关的功能性食品提供了依据。
