Trevor Lucy V, Riches-Suman Kirsten, Mahajan Ajay L, Thornton M Julie
Plastic Surgery and Burns Research Unit, University of Bradford, Bradford BD7 1DP, UK.
Department of Plastic and Reconstructive Surgery, Bradford Teaching Hospitals NHS Foundation Trust, Bradford BD9 6RJ, UK.
J Clin Med. 2023 Mar 4;12(5):2052. doi: 10.3390/jcm12052052.
Beneficial effects have been observed following the transplant of lipoaspirates containing adipose-derived stem cells into chronic wounds caused by oncologic radiotherapy. It is not yet certain whether adipose-derived stem cells are resistant to radiation exposure. Therefore, the aims of this study were to isolate stromal vascular fraction from human breast tissue exposed to radiotherapy and determine the presence of adipose-derived stem cells. Stromal vascular fraction from irradiated donor tissue was compared to commercially sourced pre-adipocytes. Immunocytochemistry was used to determine the presence of adipose-derived stem cell markers. Conditioned media from stromal vascular fraction isolated from irradiated donors was used as a treatment in a scratch wound assay of dermal fibroblasts also isolated from irradiated donors and compared to pre-adipocyte conditioned media and serum free control. This is the first report of human stromal vascular fraction being cultured from previously irradiated breast tissue. Stromal vascular fraction conditioned media from irradiated donors had a similar effect in increasing the migration of dermal fibroblasts from irradiated skin to pre-adipocyte conditioned media from healthy donors. Therefore, the ability of adipose-derived stem cells in the stromal vascular fraction to stimulate dermal fibroblasts in wound healing appears to be preserved following radiotherapy. This study demonstrates that stromal vascular fraction from irradiated patients is viable, functional and may have potential for regenerative medicine techniques following radiotherapy.
将含有脂肪来源干细胞的脂肪抽吸物移植到肿瘤放疗引起的慢性伤口后，已观察到有益效果。脂肪来源干细胞是否对辐射暴露具有抗性尚不确定。因此，本研究的目的是从接受放疗的人乳腺组织中分离基质血管成分，并确定脂肪来源干细胞的存在。将来自受辐照供体组织的基质血管成分与商业来源的前脂肪细胞进行比较。采用免疫细胞化学法检测脂肪来源干细胞标志物的存在。将来自受辐照供体分离的基质血管成分的条件培养基用于同样从受辐照供体分离的真皮成纤维细胞的划痕试验中作为处理，并与前脂肪细胞条件培养基和无血清对照进行比较。这是首次报道从先前接受过辐照的乳腺组织中培养出人基质血管成分。来自受辐照供体的基质血管成分条件培养基在增加真皮成纤维细胞从受辐照皮肤向来自健康供体的前脂肪细胞条件培养基迁移方面具有类似作用。因此，放疗后，基质血管成分中的脂肪来源干细胞在伤口愈合中刺激真皮成纤维细胞的能力似乎得以保留。本研究表明，来自受辐照患者的基质血管成分是有活力的、有功能的，并且在放疗后可能具有再生医学技术的潜力。
