Department of Pharmaceutical Botany, Faculty of Pharmacy and Biochemistry, University of Zagreb, Kovačićeva 1, 10000 Zagreb, Croatia.
Mutagenesis Unit, Institute form Medical Research and Occupational Health, Ksaverska c. 2, 10000 Zagreb, Croatia.
Molecules. 2023 Feb 22;28(5):2046. doi: 10.3390/molecules28052046.
The aim of this study was to test the phytotoxicity and mode of action of bisphenol A (BPA) on using a multibiomarker approach. roots were exposed to BPA in concentration range 0-50 mg L for 3 days. BPA even in the lowest applied concentration (1 mg L) reduced root length, root fresh weight, and mitotic index. Additionally, the lowest BPA concentration (1 mg L) decreased the level of gibberellic acid (GA) in root cells. BPA at concentration 5 mg L increased production of reactive oxygen species (ROS) that was followed by increase in oxidative damage to cells' lipids and proteins and activity of enzyme superoxide dismutase. BPA in higher concentrations (25 and 50 mg L) induced genome damage detected as an increase in micronucleus (MNs) and nuclear buds (NBUDs). BPA at >25 mg L induced synthesis of phytochemicals. Results of this study using multibiomarker approach indicate that BPA is phytotoxic to roots and has shown genotoxic potential to plants, thus its presence in the environment should be monitored.
本研究旨在采用多生物标志物方法测试双酚 A（BPA）对 的植物毒性和作用模式。将 根暴露于浓度范围为 0-50mg/L 的 BPA 中 3 天。即使在最低应用浓度（1mg/L）下，BPA 也会降低根长、根鲜重和有丝分裂指数。此外，最低浓度的 BPA（1mg/L）降低了根细胞中赤霉素（GA）的水平。BPA 在 5mg/L 的浓度下会增加活性氧物质（ROS）的产生，随后会导致细胞脂质和蛋白质的氧化损伤增加，以及超氧化物歧化酶的活性增加。较高浓度（25mg/L 和 50mg/L）的 BPA 会诱导基因组损伤，表现为微核（MNs）和核芽（NBUDs）的增加。BPA 浓度高于 25mg/L 时会诱导植物次生代谢产物的合成。本研究采用多生物标志物方法的结果表明，BPA 对 根具有植物毒性，并显示出对植物的遗传毒性潜力，因此应监测其在环境中的存在。
