Department of Plant Product Technology and Nutrition Hygiene, Faculty of Food Technology, University of Agriculture in Krakow, Al. Mickiewicza 21, 31-120 Krakow, Poland.
Department of Organic Chemistry and Technology, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24, 31-155 Krakow, Poland.
Molecules. 2023 Mar 4;28(5):2365. doi: 10.3390/molecules28052365.
Elderflower extracts are known to be a source of valuable substances that show a wide spectrum of biological activity, including antibacterial and antiviral properties, which demonstrate a degree of effectiveness against SARS CoV-2. In this work, the influence of fresh inflorescence stabilisation methods (freezing, air drying, and lyophilisation) and extraction parameters on the composition and antioxidant properties of the extracts were studied. Wild elderflower plants growing in the Małopolska Region of Poland were studied. Antioxidant activities were evaluated by 2,2-diphenyl-1-picrylhydrazyl free radical-scavenging ability and ferric-reducing antioxidant power assays. The total phenolic content was determined using the Folin-Ciocalteu method and the phytochemical profile of the extracts was analysed using HPLC. The obtained results showed that the best method for the stabilisation of elderflower was lyophilisation, and the determined optimal maceration parameters were 60% methanol as a solvent and a process time of 1-2 days.
接骨木花提取物是一种具有多种生物活性的有价值物质的来源,包括抗菌和抗病毒特性,对 SARS-CoV-2 表现出一定的有效性。在这项工作中,研究了新鲜花序稳定方法(冷冻、风干和冻干)和提取参数对提取物成分和抗氧化特性的影响。研究了在波兰小波兰省生长的野生接骨木花植物。通过 2,2-二苯基-1-苦基肼自由基清除能力和铁还原抗氧化能力测定法评估抗氧化活性。使用 Folin-Ciocalteu 法测定总酚含量,并使用 HPLC 分析提取物的植物化学特征。所得结果表明,稳定接骨木花的最佳方法是冻干,确定的最佳浸渍参数是 60%甲醇作为溶剂和 1-2 天的过程时间。