Bhattacharya Sumangala, Christensen Kathrine B, Olsen Louise C B, Christensen Lars P, Grevsen Kai, Færgeman Nils J, Kristiansen Karsten, Young Jette F, Oksbjerg Niels
Department of Food Science, Aarhus University , Blicher's Allé 20, Postbox 50, 8830 Tjele, Denmark.
J Agric Food Chem. 2013 Nov 20;61(46):11033-40. doi: 10.1021/jf402838a. Epub 2013 Nov 7.
Obesity and insulin resistance in skeletal muscles are major features of type 2 diabetes. In the present study, we examined the potential of Sambucus nigra flower (elderflowers) extracts to stimulate glucose uptake (GU) in primary porcine myotubes and reduce fat accumulation (FAc) in Caenorhabditis elegans. Bioassay guided chromatographic fractionations of extracts and fractions resulted in the identification of naringenin and 5-O- caffeoylquinic acid exhibiting a significant increase in GU. In addition, phenolic compounds related to those found in elderflowers were also tested, and among these, kaempferol, ferulic acid, p-coumaric acid, and caffeic acid increased GU significantly. FAc was significantly reduced in C. elegans, when treated with elderflower extracts, their fractions and the metabolites naringenin, quercetin-3-O-rutinoside, quercetin-3-O-glucoside, quercetin-3-O-5″-acetylglycoside, kaempferol-3-O-rutinoside, isorhamnetin-3-O-rutinoside, and isorhamnetin-3-O-glucoside and the related phenolic compounds kaempferol and ferulic acid. The study indicates that elderflower extracts contain bioactive compounds capable of modulating glucose and lipid metabolism, suitable for nutraceutical and pharmaceutical applications.
骨骼肌中的肥胖和胰岛素抵抗是2型糖尿病的主要特征。在本研究中，我们检测了黑接骨木花（接骨木花）提取物刺激原代猪肌管中葡萄糖摄取（GU）以及减少秀丽隐杆线虫脂肪积累（FAc）的潜力。提取物和馏分的生物测定导向色谱分离鉴定出柚皮素和5-O-咖啡酰奎尼酸，它们能显著增加葡萄糖摄取。此外，还测试了与接骨木花中发现的酚类化合物相关的化合物，其中山奈酚、阿魏酸、对香豆酸和咖啡酸能显著增加葡萄糖摄取。当用接骨木花提取物、其馏分以及代谢产物柚皮素、槲皮素-3-O-芸香糖苷、槲皮素-3-O-葡萄糖苷、槲皮素-3-O-5″-乙酰糖苷、山奈酚-3-O-芸香糖苷、异鼠李素-3-O-芸香糖苷、异鼠李素-3-O-葡萄糖苷以及相关酚类化合物山奈酚和阿魏酸处理时，秀丽隐杆线虫的脂肪积累显著减少。该研究表明，接骨木花提取物含有能够调节葡萄糖和脂质代谢的生物活性化合物，适用于营养保健品和药物应用。
