野生植物中的酚类化合物及其培养与抗氧化活性评价

Phenolic Compounds from Wild Plant and Cultures of and Evaluation of Their Antioxidant Activity.

作者信息

Motolinia-Alcántara Elizabeth Alejandra, Franco-Vásquez Adrián Marcelo, Nieto-Camacho Antonio, Arreguín-Espinosa Roberto, Rodríguez-Monroy Mario, Cruz-Sosa Francisco, Román-Guerrero Angelica

机构信息

Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Av. Ferrocarril de San Rafael Atlixco 186, Col. Leyes de Reforma 1a. Sección, Alcaldía Iztapalapa, Ciudad de Mexico 09310, Mexico.

Departamento de Química de Biomacromoléculas, Instituto de Química, Universidad Nacional Autónoma de México, Coyoacán, Ciudad de Mexico 04510, Mexico.

出版信息

Plants (Basel). 2023 Mar 1;12(5):1107. doi: 10.3390/plants12051107.

DOI:10.3390/plants12051107

PMID:36903964

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10005229/

Abstract

, is commonly used in traditional Mexican medicine. cultures were established from wild plant (WP) seeds, obtaining plant (IP), callus culture (CC), and cell suspension culture (CSC) with the objective to determine total phenol content (TPC) and flavonoids (TFC), as well as their antioxidant activity by DPPH, ABTS and TBARS assays, added to the compound's identification and quantification by HPLC, from methanol extracts obtained by sonication. CC showed significantly higher TPC and TFC than WP and IP, while CSC produced 2.0-2.7 times more TFC than WP, and IP produced only 14.16% TPC and 38.8% TFC compared with WP. There were identified compounds such as epicatechin (EPI), caffeic acid (CfA), and p-coumaric acid (pCA) in cultures that were not found in WP. The quantitative analysis shows gallic acid (GA) as the least abundant compound in samples, whereas CSC produced significantly more EPI and CfA than CC. Despite these results, cultures show lower antioxidant activity than WP, for DPPH and TBARS WP > CSC > CC > IP and ABTS WP > CSC = CC > IP. Overall, WP and cultures produce phenolic compounds with antioxidant activity, especially CC and CSC, which are shown to be a biotechnological alternative for obtaining bioactive compounds.

摘要

在墨西哥传统医学中常用。从野生植物（WP）种子建立了培养物，获得了离体培养植物（IP）、愈伤组织培养物（CC）和细胞悬浮培养物（CSC），目的是通过DPPH、ABTS和TBARS分析测定总酚含量（TPC）和黄酮类化合物（TFC），以及它们的抗氧化活性，并通过HPLC对超声处理得到的甲醇提取物中的化合物进行鉴定和定量。CC的TPC和TFC显著高于WP和IP，而CSC产生的TFC比WP多2.0 - 2.7倍，与WP相比，IP产生的TPC仅为14.16%，TFC为38.8%。在培养物中鉴定出了表儿茶素（EPI）、咖啡酸（CfA）和对香豆酸（pCA）等化合物，而在WP中未发现。定量分析表明没食子酸（GA）是样品中含量最少的化合物，而CSC产生的EPI和CfA比CC显著更多。尽管有这些结果，但对于DPPH和TBARS分析，培养物的抗氧化活性低于WP，即WP > CSC > CC > IP，对于ABTS分析，WP > CSC = CC > IP。总体而言，WP和培养物产生具有抗氧化活性的酚类化合物，特别是CC和CSC，它们被证明是获得生物活性化合物的生物技术替代方法。