Patle Tarun Kumar, Shrivas Kamlesh, Kurrey Ramsingh, Upadhyay Seema, Jangde Rajendra, Chauhan Ravishankar
School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur 492 010, Chhattisgarh, India.
School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur 492 010, Chhattisgarh, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Dec 5;242:118717. doi: 10.1016/j.saa.2020.118717. Epub 2020 Jul 20.
Here, we report an ultrasonic-assisted extraction (UAE) of phytochemicals from bark, leaves, sepals, fruits, and seeds of Dillenia pentagyna (Roxb) using different organic solvents such as chloroform, ethanol, and n-hexane. The preliminary phytochemical screening results showed that the ethanolic extract is enriched with phenolics, flavonoids, tannin, saponin, alkaloid, and terpenoids. The profiling of phytochemicals is carried out employing UV-Vis and Fourier-transform infrared (FTIR) spectroscopy analyses. The higher amount of phenolic compounds obtained in the ethanolic extract of bark and leaves as compared to other parts of the plant. Consequently, a higher amount of total flavonoid compounds unveiled in the bark of targeted species. The ethanolic extract of bark and leaves showed good free radical scavenging activity using DPPH with inhibition percentage of 90.58 ± 1.89% and 76.46 ± 1.58%, respectively, in comparison to standard ascorbic acid at 10 μg/mL. Moreover, the half-maximal inhibitory concentration (IC) value of bark and leaves are 5.64 and 6.54 μg/mL, respectively, in comparison to standard ascorbic acid. With the best of our knowledge, it is the first report pertaining to characterization and quantification of phenols and flavonoids as well as the investigation of the medicinal property in D. pentagyna.
在此，我们报告了使用氯仿、乙醇和正己烷等不同有机溶剂，对五桠果（Dillenia pentagyna (Roxb)）的树皮、树叶、萼片、果实和种子进行植物化学物质的超声辅助提取（UAE）。初步的植物化学筛选结果表明，乙醇提取物富含酚类、黄酮类、单宁、皂苷、生物碱和萜类化合物。采用紫外可见光谱和傅里叶变换红外光谱（FTIR）分析对植物化学物质进行了剖析。与植物的其他部分相比，树皮和树叶的乙醇提取物中获得的酚类化合物含量更高。因此，在目标物种的树皮中发现了更高含量的总黄酮化合物。树皮和树叶的乙醇提取物使用DPPH显示出良好的自由基清除活性，与10μg/mL的标准抗坏血酸相比，抑制率分别为90.58±1.89%和76.46±1.58%。此外，与标准抗坏血酸相比，树皮和树叶的半数抑制浓度（IC）值分别为5.64和6.54μg/mL。据我们所知，这是关于五桠果中酚类和黄酮类化合物的表征和定量以及药用特性研究的首次报道。
