Inorganic Chemistry Department, Faculty of Chemistry and Pharmacy, Pontificia Universidad Católica de Chile, Avenida Vicuña Mackenna 4860, Santiago 8330077, Chile.
Escuela de Ciencias Agrícolas y Veterinarias, Universidad Viña del Mar, Agua Santa 7055, Viña del Mar 2531015, Chile.
Molecules. 2022 Jan 18;27(3):610. doi: 10.3390/molecules27030610.
Plant biochemistry studies have increased in recent years due to their potential to improve human health. is an extremophile plant with an interesting polyphenolic profile. However, its biomass is scarce and occasionally available. in vitro biomass was obtained from tissue culture and compared with in vivo roots regarding its polyphenolic and flavonoid content. Different solvents were used to prepare extracts from the in vitro tissue of callus and aerial plant organs and in vivo roots. UPLC-MS/MS was used to assess the chemical composition of each extract. ORAC-FL and scavenging of free radicals (DPPH and OH) methods were used to determine the antioxidant capacity of extracts. Furthermore, the biological activity of the extracts was established using the cellular antioxidant activity method. The vitroplants were a good source of polyphenols (25-68 mg GAE/100 g tissue FW), and methanol was the most efficient solvent. Eight polyphenolic compounds were identified, and their antioxidant properties were investigated by different chemical methods with EPR demonstrating its specific scavenging activity against free radicals. All extracts showed cellular dose-dependent antioxidant activity. The methanolic extract of vitroplants showed the highest cellular antioxidant activity (44.6% and 51%) at 1 and 10 µg/mL of extract, respectively. Vitroplants of are proposed as a biotechnological product as a source of antioxidant compounds with multiple applications.
近年来，由于植物生物化学研究有可能改善人类健康，因此其研究有所增加。 是一种极端耐热植物，具有有趣的多酚谱。然而，其生物量稀少且偶尔可得。通过组织培养获得了 的体外生物量，并将其与体内根的多酚和类黄酮含量进行了比较。使用不同的溶剂从愈伤组织和地上植物器官和体内根的体外组织中制备提取物。使用 UPLC-MS/MS 评估每种提取物的化学成分。ORAC-FL 和自由基清除（DPPH 和 OH）方法用于测定提取物的抗氧化能力。此外，还使用细胞抗氧化活性方法确定了提取物的生物活性。体外植物是多酚的良好来源（25-68 mg GAE/100 g 组织 FW），甲醇是最有效的溶剂。鉴定了 8 种多酚化合物，并通过不同的化学方法研究了它们的抗氧化特性，EPR 证明其对自由基具有特定的清除活性。所有提取物均显示出细胞剂量依赖性的抗氧化活性。在 1 和 10 µg/mL 的提取物浓度下，体外植物的甲醇提取物分别显示出最高的细胞抗氧化活性（44.6%和 51%）。建议将 的体外植物作为具有多种应用的抗氧化化合物的生物技术产品的来源。
