State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Nutrients. 2023 Feb 24;15(5):1146. doi: 10.3390/nu15051146.
Cathartic colon (CC), a type of slow-transit constipation caused by the long-term use of stimulant laxatives, does not have a precise and effective treatment. This study aimed to evaluate the ability of CCFM1163 to relieve CC and to investigate its underlying mechanism. Male C57BL/6J mice were treated with senna extract for 8 weeks, followed by a 2-week treatment with CCFM1163. The results revealed that CCFM1163 effectively alleviated CC symptoms. The possible mechanism of CCFM1163 in relieving CC was analyzed by measuring the intestinal barrier and enteric nervous system (ENS)-related indices and establishing a correlation between each index and gut microbiota. The results indicated that CCFM1163 changed the gut microbiota by significantly increasing the relative abundance of and as well as the content of short-chain fatty acids, especially propionic acid, in the feces. This increased the expression of tight junction proteins and aquaporin 8, decreased intestinal transit time, increased fecal water content, and relieved CC. In addition, CCFM1163 also increased the relative abundance of in feces and the expression of enteric nerve marker proteins to repair the ENS, promote intestinal motility, and relieve constipation.
通便结肠（CC）是一种由长期使用刺激性泻药引起的慢传输型便秘，目前尚无确切有效的治疗方法。本研究旨在评估 CCFM1163 缓解 CC 的能力，并探讨其潜在机制。雄性 C57BL/6J 小鼠用番泻叶提取物处理 8 周，然后用 CCFM1163 处理 2 周。结果表明，CCFM1163 能有效缓解 CC 症状。通过测量肠道屏障和肠神经系统（ENS）相关指标，并建立每个指标与肠道微生物群之间的相关性，分析了 CCFM1163 缓解 CC 的可能机制。结果表明，CCFM1163 通过显著增加 和 的相对丰度以及粪便中短链脂肪酸（特别是丙酸）的含量来改变肠道微生物群。这增加了紧密连接蛋白和水通道蛋白 8 的表达，减少了肠道转运时间，增加了粪便含水量，并缓解了 CC。此外，CCFM1163 还增加了粪便中 的相对丰度和肠神经标记蛋白的表达，以修复 ENS，促进肠道蠕动，缓解便秘。
