Sugiyama M, Ando A, Furuno A, Furlong N B, Hidaka T, Ogura R
Department of Medical Biochemistry, Kurume University School of Medicine, Japan.
Cancer Lett. 1987 Dec;38(1-2):1-7. doi: 10.1016/0304-3835(87)90193-5.
The effects of vitamin E, vitamin B2 and selenite on DNA single strand breaks induced by Na2CrO4 were examined by alkaline elution. Incubation of Chinese hamster V-79 cells with alpha-tocopherol succinate (vitamin E) for 24 h prior to exposure to Na2CrO4 resulted in a decrease of DNA breaks produced by this compound. However, similar pretreatment with riboflavin (vitamin B2) or Na2SeO3 resulted in an enhanced formation of breaks induced by Na2CrO4. Pretreatment with Na2SeO3 resulted in increased levels of glutathione in these cells while levels of glutathione remained the same with vitamin E or vitamin B2. These results suggest that Na2CrO4 induced DNA breaks appear to be mediated by the formation of free radicals and/or cellular reductive metabolism.
通过碱性洗脱法研究了维生素E、维生素B2和亚硒酸盐对重铬酸钠诱导的DNA单链断裂的影响。在暴露于重铬酸钠之前，将中国仓鼠V - 79细胞与琥珀酸生育酚（维生素E）孵育24小时，导致该化合物产生的DNA断裂减少。然而，用核黄素（维生素B2）或亚硒酸钠进行类似的预处理导致重铬酸钠诱导的断裂形成增加。用亚硒酸钠预处理导致这些细胞中谷胱甘肽水平升高，而维生素E或维生素B2处理后谷胱甘肽水平保持不变。这些结果表明，重铬酸钠诱导的DNA断裂似乎是由自由基的形成和/或细胞还原代谢介导的。
