Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China.
Department of Clinical Laboratory, Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China.
Cell Mol Biol (Noisy-le-grand). 2022 Sep 30;68(9):140-145. doi: 10.14715/cmb/2022.68.9.22.
Ovarian cancer (OC) is one of the most commonly diagnosed cancers among women. Moreover, Endometrial cancer (EC) is a usual genital tract cancer in females; however, the hub genes and molecular pathways shared by these two cancers have not been surveyed yet. So, this study aimed to identify the common candidate genes or biomarkers and molecular pathways in OC and EC. Differences in the expressed genes between these two microarray data sets were detected. Pathway enrichment analysis and gene ontology (GO) was also performed and protein-protein interactions (PPI) network analysis was done using Cytoscape and the most important genes were identified by the Cytohubba plugin. We found that 154 common DEGs shared by OC and EC were also detected. 10 hub proteins were identified as follows: CDC20, BUB1, CENPF, KIF11, CCNB2, FOXM1, TTK, TOP2A, DEPDC1, and NCAPG. The most important and significant miRNAs were identified to be hsa-mir-186-5p, hsa-mir-192-5p, hsa-mir-215-5p, and hsa-mir-193b-3p regulated expressions of the DEGs. This investigation demonstrated that these hub genes and their miRNA might be key genes with great effects on OC and EC. However, more studies are needed for a better understanding of the role of these hub genes and their function in these two cancers.
卵巢癌（OC）是女性最常见的癌症之一。此外，子宫内膜癌（EC）是女性常见的生殖道癌症；然而，这两种癌症之间共享的核心基因和分子途径尚未被调查。因此，本研究旨在确定 OC 和 EC 中常见的候选基因或生物标志物和分子途径。检测了这两个微阵列数据集之间表达基因的差异。还进行了途径富集分析和基因本体（GO），并使用 Cytoscape 进行了蛋白质-蛋白质相互作用（PPI）网络分析，并使用 Cytohubba 插件确定了最重要的基因。我们发现 OC 和 EC 之间还检测到 154 个共同的差异表达基因。确定了 10 个核心蛋白，如下所示：CDC20、BUB1、CENPF、KIF11、CCNB2、FOXM1、TTK、TOP2A、DEPDC1 和 NCAPG。鉴定出最重要和最显著的 miRNA 是 hsa-mir-186-5p、hsa-mir-192-5p、hsa-mir-215-5p 和 hsa-mir-193b-3p 调节 DEGs 的表达。这项研究表明，这些核心基因及其 miRNA 可能是对 OC 和 EC 具有重大影响的关键基因。然而，需要更多的研究来更好地理解这些核心基因及其在这两种癌症中的功能。
