Alghamdi Saad, Ashshi Ahmad, Kabrah Ahmed, Atwah Banan, Alkurbi Mohammad Othman, Assas Mushref Bakri
Laboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi Arabia.
Faculty of Applied Medical Sciences, Department of Medical Laboratory Technology, Immunology Group, King Abdul Aziz University, Jeddah, Saudi Arabia.
Cell Mol Biol (Noisy-le-grand). 2022 Sep 30;68(9):45-50. doi: 10.14715/cmb/2022.68.9.7.
This study aimed to investigate the relationship between gastrointestinal tract infection and dengue hemorrhagic fever. Dengue hemorrhagic fever is a syndrome caused by the dengue virus and primarily affects children below ten years of age and is spread by the Aedes aegypti mosquito. Gastrointestinal tract infection is a bacterial and parasitic infection that leads to gastrointestinal tract inflammation which involves the small intestine and the stomach. The relationship between the two can be manifested by gastrointestinal bleeding, acute pancreatitis, and fulminant liver failure. In this research work, 600 blood and feces samples of different ages and sex (7-8 worms) were collected from Jeddah city. From the blood samples, serum was made and stored at -20°C until use. The frozen sera samples were investigated for sero-detection of DENV-NS1 antigen as a rapid, sensitive, and cost-effective test to detect asymptomatic acute DENV-infected donors and anti-DENV IgM and IgG antibodies. Feces samples were processed for the detection of parasites. The data acquired from these samples of all the 600 participants were analyzed and interpreted, followed by statistical analysis using GraphPad Prism 5.0 software. All the values were considered significant, which showed a value of less than 0.05. Results were expressed as with the range. This article documents that gastrointestinal tract manifestations frequently occur among patients with dengue hemorrhagic fever. There are close relationships between gastrointestinal tract infection and dengue hemorrhagic fever. In current work, it was established that dengue fever leads to gastrointestinal tract bleeding in the presence of intestinal parasites. Therefore, failure to identify the patients with this infection early enough can lead to an increased morbidity and mortality rate.
本研究旨在调查胃肠道感染与登革出血热之间的关系。登革出血热是一种由登革病毒引起的综合征，主要影响10岁以下儿童，由埃及伊蚊传播。胃肠道感染是一种细菌和寄生虫感染，会导致胃肠道炎症，累及小肠和胃。两者之间的关系可表现为胃肠道出血、急性胰腺炎和暴发性肝衰竭。在这项研究工作中，从吉达市收集了600份不同年龄和性别的血液和粪便样本（7 - 8份样本）。从血液样本中制备血清，并在-20°C下储存直至使用。对冷冻血清样本进行登革病毒非结构蛋白1（DENV-NS1）抗原的血清学检测，这是一种快速、灵敏且经济高效的检测无症状急性登革病毒感染献血者以及抗登革病毒IgM和IgG抗体的检测方法。粪便样本用于寄生虫检测。对所有600名参与者的这些样本所获取的数据进行分析和解读，随后使用GraphPad Prism 5.0软件进行统计分析。所有数值被认为具有显著性，即显示值小于0.05。结果以范围表示。本文记录了登革出血热患者中胃肠道表现频繁出现。胃肠道感染与登革出血热之间存在密切关系。在当前研究中，已证实登革热在存在肠道寄生虫的情况下会导致胃肠道出血。因此，未能足够早地识别出这种感染的患者会导致发病率和死亡率上升。
