Department of Biochemistry, University of Dschang, Dschang, Cameroon.
Public School of Medical Laboratory Technicians, Yaoundé, Cameroon.
Pan Afr Med J. 2020 Jul 14;36:185. doi: 10.11604/pamj.2020.36.185.22128. eCollection 2020.
the risk of dengue virus or its antibodies which can be transmitted through blood transfusion by asymptomatic individuals infected, has been a major concern all over the world. Dengue is an endemic disease in sub-Saharan Africa, particularly in Cameroon. The purpose of this study is to determine the frequency of dengue virus (DENV) infection among potential blood donors at Yaounde Jamot Hospital.
serum samples were collected from 310 potential adult blood donors aged 18-57 years, who signed a written informed consent and completed the questionnaire between March 2019 and August 2019. This serum is used to screen for the presence of serological markers of DENV infection (NS1, IgM and IgG) using immunochromatographic tests (Zhuhai Encode Medical Engineering Co., Ltd, China). IgM/IgG positive samples were confirmed by enzyme-linked immunosorbent assays (ELISA).
the overall prevalence was 24.8% among potential blood donors were subdivided as follows: 4.5% (14/310), 12.3% (38/310) and 6.1% (19/310) showed mono-positivity to DENV-NS1 antigen, anti-DENV IgM and anti-DENV IgG antibodies respectively. 1.9% (6/310) of potential blood donors showed dual positivity to anti-DENV IgM antibodies and anti-DENV IgG antibodies. The presence of DENV-NS1 antigen show asymptomatic viremia of dengue at the time of donation, while the presence of IgG antibodies reflects the high endemicity of dengue disease in the city of Yaoundé.
these findings demonstrate the high level of risk of the DENV transmission among potential blood donors to needy recipients, underscoring the importance of establishing dengue fever blood screening in different services and blood collection units in Cameroon to improve safety transfusion and control the dissemination of the DENV.
无症状感染者通过输血传播登革热病毒或其抗体的风险一直是全世界关注的主要问题。登革热是撒哈拉以南非洲的地方性疾病，特别是在喀麦隆。本研究的目的是确定雅温得雅穆特医院潜在献血者中登革热病毒（DENV）感染的频率。
收集了 310 名年龄在 18-57 岁之间的成年潜在献血者的血清样本，他们签署了书面知情同意书并在 2019 年 3 月至 2019 年 8 月期间完成了问卷。使用免疫层析试验（珠海亿胜生物制药有限公司，中国）检测血清中登革热感染的血清学标志物（DENV-NS1、IgM 和 IgG）的存在。对 IgM/IgG 阳性样本进行酶联免疫吸附试验（ELISA）确认。
潜在献血者的总体患病率为 24.8%，具体如下：DENV-NS1 抗原、抗 DENV IgM 和抗 DENV IgG 抗体单阳性分别为 4.5%（14/310）、12.3%（38/310）和 6.1%（19/310）。6.1%（19/310）的潜在献血者对 DENV-NS1 抗原和抗 DENV IgG 抗体呈双阳性。DENV-NS1 抗原的存在表明在献血时存在无症状登革热病毒血症，而 IgG 抗体的存在反映了雅温得市登革热疾病的高度地方性。
这些发现表明，潜在献血者中存在 DENV 传播的高风险，这使得需要接受输血的患者处于危险之中，这突显了在喀麦隆不同服务和血液采集单位建立登革热血液筛查的重要性，以提高输血安全性并控制 DENV 的传播。
