Department of Parasitology, Leiden University Medical Centre, Leiden, Netherlands.
Methods Mol Biol. 2023;2618:219-237. doi: 10.1007/978-1-0716-2938-3_16.
In response to different stimuli, dendritic cells (DCs) undergo metabolic reprogramming to support their function. Here we describe how fluorescent dyes and antibody-based approaches can be used to assess various metabolic parameters of DCs including glycolysis, lipid metabolism, mitochondrial activity, and the activity of important sensors and regulators of cellular metabolism, mTOR and AMPK. These assays can be performed using standard flow cytometry and will allow for the determination of metabolic properties of DC populations at single-cell level and to characterize metabolic heterogeneity within them.
针对不同的刺激，树突状细胞 (DCs) 会经历代谢重编程以支持其功能。在这里，我们描述了如何使用荧光染料和基于抗体的方法来评估 DCs 的各种代谢参数，包括糖酵解、脂质代谢、线粒体活性以及细胞代谢的重要传感器和调节剂 mTOR 和 AMPK 的活性。这些测定可以使用标准流式细胞术进行，并允许在单细胞水平上确定 DC 群体的代谢特性，并描述它们内部的代谢异质性。
