College of Food Science and Engineering, Bohai University, Jinzhou, 121013, China.
College of Food Science and Engineering, Bohai University, Jinzhou, 121013, China; National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Jinzhou, 121013, China.
Fungal Biol. 2023 Mar;127(3):949-957. doi: 10.1016/j.funbio.2023.02.001. Epub 2023 Feb 9.
Black spot rot caused by Alternaria alternata is one of the major postharvest disease of apple fruit during logistic. This study evaluated in vitro inhibitory effect of 2-hydroxy-3-phenylpropanoic acid (PLA) at various concentrations on A. alternata and the possible mechanisms involved in its action. Results showed that different concentrations of PLA inhibited conidia germination and mycelial growth of A. alternata in vitro, and 1.0 g L was the lowest effective concentration to suppress A. alternata growth. Moreover, PLA significantly reduced relative conductivity and increased malondialdehyde and soluble protein contents. PLA also increased HO and dehydroascorbic acid contents, but reduced ascorbic acid content. Additionally, PLA treatment inhibited catalase, ascorbate peroxidase, monodehydroascorbate acid reductase, dehydroascorbic acid reductase and glutathione reductase activities, whereas promoted superoxide dismutase activity. All these findings suggest that the possible mechanisms involved in the inhibitory effect of PLA on A. alternata included damaging the cell membrane integrity to cause electrolyte leakage and destroying reactive oxygen species balance.
黑斑病是苹果果实采后物流过程中的主要病害之一，由交链格孢菌引起。本研究评估了 2-羟基-3-苯丙酸（PLA）在不同浓度下对交链格孢菌的体外抑制作用及其作用机制。结果表明，不同浓度的 PLA 抑制了交链格孢菌的孢子萌发和菌丝生长，1.0 g/L 是抑制交链格孢菌生长的最低有效浓度。此外，PLA 显著增加了丙二醛和可溶性蛋白含量，降低了相对电导率。PLA 还增加了 H2O2 和脱氢抗坏血酸的含量，降低了抗坏血酸的含量。此外，PLA 处理抑制了过氧化氢酶、抗坏血酸过氧化物酶、单脱氢抗坏血酸还原酶、脱氢抗坏血酸还原酶和谷胱甘肽还原酶的活性，而促进了超氧化物歧化酶的活性。这些结果表明，PLA 对交链格孢菌的抑制作用可能包括破坏细胞膜完整性导致电解质泄漏和破坏活性氧平衡。
