Xing Daochao, Cheng Jingguang, Song Xiefa
Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China.
Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China.
Environ Res. 2023 Jun 1;226:115636. doi: 10.1016/j.envres.2023.115636. Epub 2023 Mar 11.
The sodium alginate-HBO (SA-HBO) is traditionally used as bioremediation method for wastewater treatment in recirculating aquaculture system. Even though this method has many advantages (e.g., high cell loading) for immobilization, the remove of ammonium is not very effective. In this study, a modified method was built by adding polyvinyl alcohol and activated carbon into SA solution, and then crosslinked with saturated HBO-CaCl solution for creating new beads. Moreover, response surface methodology was utilized for optimizing the immobilization based on Box-Behnken design. The removal rate of ammonium in 96 h was taken as the primary performance criterion to characterize the biological activity of immobilized microorganisms (i.e., Chloyella pyrenoidosa, Spirulina platensis, Nitrifying bacteria, and Photosynthetic bacteria). Based on the results, the optimal parameter of immobilization as follows: the concentration of SA was 1.46%, the concentration of polyvinyl alcohol was 0.23%, the concentration of activated carbon was 0.11%, the crosslinking time was 29.33 h, and the pH was 6.6.
海藻酸钠-嗜硼菌（SA-HBO）传统上用作循环水养殖系统中废水处理的生物修复方法。尽管该方法在固定化方面有许多优点（例如，高细胞负载量），但去除铵的效果不是很好。在本研究中，通过向SA溶液中添加聚乙烯醇和活性炭构建了一种改进方法，然后用饱和HBO-CaCl溶液交联以制备新的珠子。此外，基于Box-Behnken设计，利用响应面法优化固定化过程。以96小时内铵的去除率作为表征固定化微生物（即，蛋白核小球藻、钝顶螺旋藻、硝化细菌和光合细菌）生物活性的主要性能标准。根据结果，固定化的最佳参数如下：SA浓度为1.46%，聚乙烯醇浓度为0.23%，活性炭浓度为0.11%，交联时间为29.33小时，pH为6.6。
