Laboratoire de Santé Animale du Québec, Ministère de l'Agriculture, des Pêcheries et de l'Alimentation du Québec, Saint-Hyacinthe, Québec, Canada.
Groupe de recherche sur les maladies infectieuses en production animale (GREMIP), Département de pathologie et microbiologie, Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada.
J Vet Diagn Invest. 2023 Nov;35(6):625-629. doi: 10.1177/10406387231160767. Epub 2023 Mar 12.
Beekeeping plays a crucial role in biodiversity, pollination, commercial farming, and the worldwide agricultural economy. Histopathology, which is an important tool for the investigation of diseases in vertebrates, is not commonly used in honey bees (). However, histopathology could potentially help the diagnostic investigation of high mortality in bees. We developed a tissue fixation and processing method enabling systematic production of histologic slides adequate for diagnostic and research purposes. Our method uses inexpensive, accessible products and can be realized with conventional pathology laboratory equipment. The quality of histologic slides obtained is similar to those of vertebrate animals processed routinely in pathology laboratories. Histopathology as a diagnostic and research tool will improve the services currently offered to apiarists and could help decrease the mean mortality rate, increase apiarists' profits, and ensure long-term pollination services.
养蜂在生物多样性、授粉、商业农业和全球农业经济中起着至关重要的作用。组织病理学是研究脊椎动物疾病的重要工具，但在蜜蜂中并不常用（）。然而，组织病理学可能有助于对蜜蜂高死亡率进行诊断性调查。我们开发了一种组织固定和处理方法，能够系统地制作用于诊断和研究目的的组织学幻灯片。我们的方法使用廉价、易于获得的产品，并可以使用常规病理实验室设备来实现。获得的组织学幻灯片的质量与在病理实验室中常规处理的脊椎动物相似。组织病理学作为一种诊断和研究工具，将提高目前向养蜂人提供的服务水平，并有助于降低平均死亡率、增加养蜂人的利润，确保长期的授粉服务。
