Bunsap Peerasak, Senarat Sinlapachai, Niyomdecha Seree, Pornsuriya Chaninun, Kaneko Gen, Thaochan Narit
Agricultural Innovation and Management Division, Faculty of Natural Resources, Prince of Songkla University, Songkhla 90110, Thailand.
Center of Excellence on Agricultural Biotechnology (AG-BIO/MHESI), Bangkok 10900, Thailand.
Insects. 2024 Jul 26;15(8):565. doi: 10.3390/insects15080565.
The brown planthopper (BPH), (Stål, 1854) is a pest of rice plants worldwide. is a destructive entomopathogenic fungus known to attack leafhoppers or BPHs specifically. Live adult BPH samples were inoculated with isolated PSUC002, and their interaction was morpho-histologically examined from 0 to 120 h post-inoculation (pi). We observed that the mortality of BPH continuously increased until 120 h pi (Day 5). Tissue alterations in the host were examined after infection using morphological and histological methods, including the Grocott Methenamine Silver stain test (GMS). Filamentous fungi were first found on the external integument at 12 h pi, and fungal conidia attached to the integument at 24 h pi. However, the initial degeneration of BPHs was identified by histology at 6 h pi especially in the integument and adipose tissue. We identified the degeneration and loss of integument and adipose tissue of infected BPHs at 12 h pi, and their necrosis was completed at 96 h pi. The enzymatic index of the sampled fungi (chitinase and protease) peaked at 7 days of incubation. This study demonstrated that PSUC002 is useful to control the BPHs as an eco-friendly practice and will possibly be applied in agriculture.
褐飞虱（BPH），（斯塔尔，1854年）是一种全球范围内危害水稻植株的害虫。绿僵菌是一种已知专门攻击叶蝉或褐飞虱的具有破坏性的昆虫病原真菌。将分离得到的PSUC002接种到活的成年褐飞虱样本上，并在接种后0至120小时（pi）对它们的相互作用进行形态组织学检查。我们观察到，褐飞虱的死亡率持续上升，直至接种后120小时（第5天）。在感染后，使用包括Grocott六胺银染色试验（GMS）在内的形态学和组织学方法检查宿主中的组织变化。丝状真菌在接种后12小时首次在体表被发现，并且在接种后24小时真菌分生孢子附着在体表。然而，褐飞虱的初始退化在接种后6小时通过组织学被识别，特别是在体表和脂肪组织中。我们在接种后12小时确定了受感染褐飞虱体表和脂肪组织的退化和丧失，并且它们的坏死在接种后96小时完成。所采样真菌的酶活性指标（几丁质酶和蛋白酶）在培养7天时达到峰值。这项研究表明，PSUC002作为一种生态友好的措施对于控制褐飞虱是有用的，并且可能会应用于农业。
