血浆中D(-)乳酸的比色测定

Colorimetric measurement of D(-)lactate in plasma.

作者信息

Buttery J E, Pannall P R

机构信息

Department of Clinical Chemistry, Queen Elizabeth Hospital, South Australia.

出版信息

Clin Biochem. 1987 Aug;20(4):237-9. doi: 10.1016/s0009-9120(87)80004-8.

DOI:10.1016/s0009-9120(87)80004-8

PMID:3690825

Abstract

We describe a method for the direct assay of D(-)lactate in plasma using D(-)lactate dehydrogenase and a color reagent to produce a formazan color measured at 510 nm. This method agrees well with a modified lactate UV end-point method r = 0.998 and the regression equation y = 0.974x + 0.13. The precision for low and high concentrations of D(-)lactate is 4.8% and 3.6% within-batch and 8.9% and 1.7% between-batch. Recoveries average 105% and 98% for D(-)lactate at concentrations of 1 and 5 mmol/L, respectively. The proposed method is inexpensive and suitable for routine and emergency use, and shares reagents used for the assay of L(+)lactate and 3-hydroxybutyrate.

摘要

我们描述了一种使用D(-)乳酸脱氢酶和一种显色试剂直接测定血浆中D(-)乳酸的方法，该试剂可产生一种在510nm处测定的甲臜颜色。此方法与改良的乳酸紫外终点法高度吻合（r = 0.998，回归方程y = 0.974x + 0.13）。低浓度和高浓度D(-)乳酸的批内精密度分别为4.8%和3.6%，批间精密度分别为8.9%和1.7%。浓度为1和5 mmol/L时，D(-)乳酸的回收率平均分别为105%和98%。所提出的方法成本低廉，适用于常规和急诊检测，且与用于L(+)乳酸和3-羟基丁酸检测的试剂相同。