Matsue H, Takagaki K, Honda K, Nakagawa Y, Gejyo F, Arakawa M, Endo M
Department of Biochemistry, Hirosaki University School of Medicine, Japan.
Clin Chem. 1987 Dec;33(12):2214-9.
We prepared human urinary glycopeptides from the supernatant liquid remaining after precipitation of the nondialyzable fraction with cetylpyridinium chloride. Using cation exchange and affinity chromatographies and gel filtration, we obtained 28 glycopeptide subfractions. By compositional analyses of sugar and amino acid, and by reducing-terminal analyses after reduction with NaBH4, we determined the size of the carbohydrate moiety and the types of carbohydrate-peptide linkage involved. We isolated several glycopeptides not previously described: six with sialic acid, two with fucose, two with glucose, and one with N-acetylgalactosamine. The sialic acid glycopeptides had a short carbohydrate chain of the O-glycoside type. The fucose-containing glycopeptides were fucosyllactosaminoglycans. The glucose glycopeptides were polymers linked to a small peptide moiety. The N-acetylgalactosamine-rich glycopeptide was found in an N-glycoside-type fraction, with N-acetylgalactosamine at the nonreducing terminal.
我们从用十六烷基氯化吡啶沉淀不可透析部分后剩余的上清液中制备了人尿糖肽。通过阳离子交换、亲和色谱和凝胶过滤，我们获得了28个糖肽亚组分。通过对糖和氨基酸的组成分析，以及在用硼氢化钠还原后的还原末端分析，我们确定了碳水化合物部分的大小以及所涉及的碳水化合物 - 肽连接类型。我们分离出了几种以前未描述过的糖肽：六种含有唾液酸，两种含有岩藻糖，两种含有葡萄糖，一种含有N - 乙酰半乳糖胺。唾液酸糖肽具有O - 糖苷型的短碳水化合物链。含岩藻糖的糖肽是岩藻糖基乳糖胺聚糖。葡萄糖糖肽是与小肽部分相连的聚合物。富含N - 乙酰半乳糖胺的糖肽存在于N - 糖苷型部分中，在非还原末端带有N - 乙酰半乳糖胺。
