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大豆皮复合膳食纤维对重组肉制品中肌原纤维蛋白凝胶特性的影响及其作用机制

Effect of compound dietary fiber of soybean hulls on the gel properties of myofibrillar protein and its mechanism in recombinant meat products.

作者信息

Zhang Song-Shan, Duan Jun-Ya, Zhang Teng-Teng, Lv Meng, Gao Xiao-Guang

机构信息

Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

College of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang, Hebei, China.

出版信息

Front Nutr. 2023 Feb 23;10:1129514. doi: 10.3389/fnut.2023.1129514. eCollection 2023.

Abstract

Response surface methodology was used to determine the optimum ratio of rice husk dietary fiber, soybean hull dietary fiber, and inulin as 1.40, 1.42, and 3.24%. The effects of compound and single dietary fiber on water holding capacity, gel strength, secondary structure, rheological properties, chemical action force, and microstructure of myofibrillar proteins (MP) gel were investigated. The application of composite dietary fiber significantly ( < 0.05) improved the gel strength, water holding capacity and storage modulus (G') of MP gel. Fourier transform infrared spectrum analysis shows that the addition of compound dietary fiber can make the gel structure more stable. The effect of dietary fiber complex on the chemical action of MP gel was further studied, and it was found that hydrophobic interaction and disulfide bond could promote the formation of compound gel. By comparing the microstructure of the MP gel with and without dietary fiber, the results showed that the MP gel with compound dietary fiber had smaller pores and stronger structure. Therefore, the rice hull dietary fiber, the soybean hull dietary fiber and the inulin are compounded and added into the low-fat recombinant meat product in a proper proportion, so that the quality characteristics and the nutritional value of the low-fat recombinant meat product can be effectively improved, the rice hull dietary fiber has the potential of being used as a fat substitute, and a theoretical basis is provided for the development of the functional meat product.

摘要

采用响应面法确定稻壳膳食纤维、大豆皮膳食纤维和菊粉的最佳比例分别为1.40%、1.42%和3.24%。研究了复合膳食纤维和单一膳食纤维对肌原纤维蛋白(MP)凝胶保水性、凝胶强度、二级结构、流变学特性、化学作用力和微观结构的影响。复合膳食纤维的应用显著(<0.05)提高了MP凝胶的凝胶强度、保水性和储能模量(G')。傅里叶变换红外光谱分析表明,复合膳食纤维的添加可使凝胶结构更稳定。进一步研究了膳食纤维复合物对MP凝胶化学作用的影响,发现疏水相互作用和二硫键可促进复合凝胶的形成。通过比较添加和未添加膳食纤维的MP凝胶的微观结构,结果表明添加复合膳食纤维的MP凝胶孔径更小、结构更强。因此,将稻壳膳食纤维、大豆皮膳食纤维和菊粉按适当比例复合添加到低脂重组肉制品中,可有效改善低脂重组肉制品的品质特性和营养价值,稻壳膳食纤维具有作为脂肪替代品的潜力,为功能性肉制品的开发提供了理论依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d86/9996004/3fd3821336cc/fnut-10-1129514-g001.jpg

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