Xiong Tian-Han, Shi Ce, Mu Chang-Kao, Wang Chun-Lin, Ye Yang-Fang
School of Marine Sciences, Ningbo University, Ningbo, China.
Key Laboratory of Aquacultural Biotechnology, Ministry of Education, Ningbo University, Ningbo, China.
Front Nutr. 2023 Feb 24;10:1092573. doi: 10.3389/fnut.2023.1092573. eCollection 2023.
Microbial community may systematically promote the development of fermentation process of foods. Traditional fermentation is a spontaneous natural process that determines a unique nutritional characteristic of crab paste of , However, rare information is available regarding the development pattern and metabolic role of bacterial community during the fermentation of crab paste. Here, using a 16S rRNA gene amplicon sequencing technology, we investigated dynamics of bacterial community and its relationship with metabolites during the fermentation of crab paste. The results showed that bacterial community changed dynamically with the fermentation of crab paste which highlighted by consistently decreased α-diversity and overwhelming dominance of at the later days of fermentation. had a positive correlation with trimethylamine, hypoxanthine, formate, and alanine while a negative correlation with inosine and adenosine diphosphate. In contrast, most of other bacterial indicators had a reverse correlation with these metabolites. Moreover, presented an improved function potential in the formation of the significantly increased metabolites. These findings demonstrate that the inexorable rise of not only drives the indicator OTUs turnover in the bacterial community but also has incriminated the quality of crab paste from fresh to perished.
微生物群落可能会系统性地促进食品发酵过程的发展。传统发酵是一个自发的自然过程,它决定了蟹酱独特的营养特性。然而,关于蟹酱发酵过程中细菌群落的发展模式和代谢作用的信息却很少。在这里,我们使用16S rRNA基因扩增子测序技术,研究了蟹酱发酵过程中细菌群落的动态变化及其与代谢产物的关系。结果表明,随着蟹酱发酵,细菌群落动态变化,其特征是α多样性持续下降,且在发酵后期 占绝对优势。 与三甲胺、次黄嘌呤、甲酸和丙氨酸呈正相关,与肌苷和二磷酸腺苷呈负相关。相比之下,大多数其他细菌指标与这些代谢产物呈相反的相关性。此外, 在显著增加的代谢产物形成中表现出增强的功能潜力。这些发现表明, 的必然增加不仅推动了细菌群落中指示性操作分类单元的更替,还影响了蟹酱从新鲜到变质的质量。