Laboratory of Biomass and Bioprocessing Engineering, College of Engineering, China Agricultural University, Beijing 100083, China.
Laboratory of Biomass and Bioprocessing Engineering, College of Engineering, China Agricultural University, Beijing 100083, China.
Bioresour Technol. 2019 Jun;282:69-74. doi: 10.1016/j.biortech.2019.02.104. Epub 2019 Feb 23.
Lignocellulosic biomass from corn stover holds promise as a raw material for the production of alternative energy to replace fossil fuels. In this study, structural properties of corn stover after pretreatment using mechanical pulverization with or without subsequent phosphoric acid treatment were investigated. The results showed that a pulverization step loosened the compact structure of corn stover lignocellulose and effectively reduced particle size, while both pulverization and phosphoric acid pretreatment steps altered the crystallinity index. During pretreatment, hemicellulose content was reduced and accessibility of β-1,4 glycosidic bonds to hydrolysis by cellulase increased, while almost all lignin was retained. The results showed that the combined two-step pretreatment method improved sugar yield from lignocellulose during subsequent enzymatic hydrolysis from 20.01 mg/g to 41.41 mg/g in glucose yield. These results should guide the development of methods for improved lignocellulose conversion to sugars for enhanced bioethanol production.
玉米秸秆中的木质纤维素生物质有望成为替代化石燃料的替代能源的原料。在这项研究中，研究了使用机械粉碎预处理玉米秸秆后，以及随后是否进行磷酸处理，其结构性能的变化。结果表明，粉碎步骤可以疏松玉米秸秆木质纤维素的致密结构，有效地减小粒径，而粉碎和磷酸预处理两个步骤都改变了结晶度指数。在预处理过程中，半纤维素含量减少，β-1,4 糖苷键对纤维素酶水解的可及性增加，而几乎所有的木质素都被保留下来。结果表明，两步联合预处理方法提高了后续酶解过程中木质纤维素的糖得率，从葡萄糖产量的 20.01 mg/g 提高到 41.41 mg/g。这些结果应该为改进木质纤维素转化为糖以提高生物乙醇产量的方法提供指导。
