用于检测芽生菌病抗体的比较斑点酶联免疫吸附测定法的开发。

Development of a comparative dot ELISA for the detection of antibodies in blastomycosis.

作者信息

Sato H, Honigman A S, Scalarone G M

机构信息

Department of Biological Sciences, Idaho State University, Pocatello 83209.

出版信息

Diagn Microbiol Infect Dis. 1987 May;7(1):37-43. doi: 10.1016/0732-8893(87)90067-8.

DOI:10.1016/0732-8893(87)90067-8

PMID:3691032

Abstract

We present a preliminary study utilizing an enzyme immunoassay for the detection of antibodies in blastomycosis using nitrocellulose membrane as the solid support. The comparative dot enzyme-linked immunosorbent assay (CDE) utilizing Blastomyces dermatitidis, Histoplasma capsulatum, and Coccidioides immitis antigens allows rapid screening of specimens and determination of a serum profile with respect to anti-Blastomyces antibodies. Sensitivity (76% or greater) and specificity (approximately 12% crossreactivity) determinations indicated that this visual assay may be an alternative antibody detection method for blastomycosis or other systemic fungal diseases.

摘要

我们展示了一项初步研究，该研究利用酶免疫测定法，以硝酸纤维素膜作为固相支持物来检测芽生菌病中的抗体。利用皮炎芽生菌、荚膜组织胞浆菌和粗球孢子菌抗原的比较斑点酶联免疫吸附测定（CDE）能够快速筛查标本，并确定血清中抗芽生菌抗体的情况。敏感性（76%或更高）和特异性（约12%的交叉反应性）测定表明，这种直观的测定法可能是用于芽生菌病或其他系统性真菌疾病的一种替代性抗体检测方法。