皮炎芽生菌酵母相裂解物抗原的制备及酶联免疫吸附测定评估

Preparation and ELISA evaluation of Blastomyces dermatitidis yeast phase lysate antigens.

作者信息

Johnson S M, Scalarone G M

机构信息

Department of Biological Sciences, Idaho State University, Pocatello 83209.

出版信息

Diagn Microbiol Infect Dis. 1988 Oct;11(2):81-6. doi: 10.1016/0732-8893(88)90076-4.

DOI:10.1016/0732-8893(88)90076-4

PMID:3229098

Abstract

Enzyme-linked immunosorbent assay (ELISA) comparative studies are described in which 10 Blastomyces dermatitidis antigenic preparations from human, dog, and soil strains were used for the detection of rabbit serum antibodies directed against B. dermatitidis killed yeast cells of human and dog origin as well as detection of antibodies in human serum samples. Reactive reagents were produced from all 10 strains, but two antigens (K-Le and T-043324) demonstrated exceptional sensitivity with moderate cross-reactivity with histoplasmosis specimens. Additionally, these reagents were able to detect anti-B. dermatitidis antibodies produced in rabbits in response to human (H-100) or dog (T-042976) strains in a comparable manner. These data suggest that a single antigenic reagent might be suitable for ELISA detection of B. dermatitidis antibodies in different species with blastomycosis.

摘要

本文描述了酶联免疫吸附测定（ELISA）比较研究，其中使用了来自人、狗和土壤菌株的10种皮炎芽生菌抗原制剂，用于检测兔血清中针对人源和狗源皮炎芽生菌死酵母细胞的抗体，以及检测人血清样本中的抗体。所有10种菌株均产生了反应性试剂，但两种抗原（K-Le和T-043324）表现出极高的敏感性，与组织胞浆菌病标本有中等程度的交叉反应。此外，这些试剂能够以类似方式检测兔体内针对人（H-100）或狗（T-042976）菌株产生的抗皮炎芽生菌抗体。这些数据表明，单一抗原试剂可能适用于ELISA检测不同物种芽生菌病患者体内的皮炎芽生菌抗体。