Garrett Stephen R, Mariano Giuseppina, Palmer Tracy
Microbes in Health and Disease Theme, Newcastle University Biosciences Institute, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK.
Access Microbiol. 2022 Nov 29;4(11). doi: 10.1099/acmi.0.000464.v3. eCollection 2022.
RN6390 is a commonly used laboratory strain of derived from NCTC8325. In this study, we sequenced the RN6390 genome and compared it to available genome sequences for NCTC8325. We confirmed that three prophages, Φ11, Φ12 and Φ13, which are present in NCTC8325 are absent from the genome of RN6390, consistent with the successive curing events leading to the generation of this strain. However, we noted that a separate prophage is present in RN6390 that is not found in NCTC8325. Two separate genome sequences have been deposited for the parental strain, NCTC8325. Analysis revealed several differences between these sequences, in particular, between the copy number of genes, which encode immunity proteins to the type VII secreted anti-bacterial toxin, EsaD. Single nucleotide polymorphisms were also detected in ribosomal RNA genes and genes encoding microbial surface components recognizing adhesive matrix molecules (MSCRAMM) between the two NCTC8325 sequences. Comparing each NCTC8325 sequence to other strains in the RN6390 lineage confirmed that sequence assembly errors in the earlier NCTC8325 sequence are the most likely explanation for most of the differences observed.
RN6390是一种常用的实验室菌株，源自NCTC8325。在本研究中，我们对RN6390基因组进行了测序，并将其与NCTC8325的现有基因组序列进行了比较。我们证实，NCTC8325中存在的三种原噬菌体Φ11、Φ12和Φ13在RN6390的基因组中不存在，这与导致该菌株产生的连续消除事件一致。然而，我们注意到RN6390中存在一种在NCTC8325中未发现的单独原噬菌体。亲本菌株NCTC8325已有两个独立的基因组序列存档。分析揭示了这些序列之间的几个差异，特别是在编码针对VII型分泌抗菌毒素EsaD的免疫蛋白的基因拷贝数方面。在两个NCTC8325序列之间的核糖体RNA基因和编码识别粘附基质分子的微生物表面成分（MSCRAMM）的基因中也检测到单核苷酸多态性。将每个NCTC8325序列与RN6390谱系中的其他菌株进行比较证实，早期NCTC8325序列中的序列组装错误最有可能解释所观察到的大多数差异。
