Enhanced Quinolone Resistance and Differential Expression of Efflux Pump Genes in Grown in Platelet Concentrates.

: Platelet concentrates (PCs) are used in transfusion medicine to treat bleeding disorders. , a predominant PC contaminant, has been implicated in several adverse transfusion reactions. The aim of this study was to investigate the impact of PC storage on resistance to quinolones, which are commonly used to treat infections. : Four transfusion-relevant strains (TRSs) were subjected to comparative transcriptome analyses when grown in PCs vs. trypticase soy broth (TSB). Results of these analyses revealed differentially expressed genes involved in antibiotic resistance. Of interest, the gene (encodes for the NorB efflux pump, which is implicated in quinolone resistance and is negatively regulated by MgrA) was upregulated (1.2-4.7-fold increase) in all PC-grown TRS compared to TSB cultures. Minimal Bactericidal Concentration (MBC) of ciprofloxacin and norfloxacin in PC-grown TRS compared to TSB showed increased resistance to both quinolones in PC cultures. Complementary studies with non-transfusion-relevant strains RN6390 and its and deletion mutants were conducted. MBC of ciprofloxacin and norfloxacin and RT-qPCR assays of these strains showed that not only , but also and may be involved in enhanced quinolone resistance in PC-grown . The role of in virulence was also tested using the silkworm animal model; lethal dose 50 (LD) assays revealed slightly higher virulence in larvae infected with the wild-type strain compared to the mutant. : The PC storage environment enhances quinolone resistance in and induces differential expression of efflux pump genes. Furthermore, our preliminary data of the involvement of NorB in virulence of using a silkworm model merit further investigation with other systems such as a mammal animal model. Our results provide mechanistic insights to aid clinicians in the selection of antimicrobial treatment of patients receiving transfusions of -contaminated PCs.