Pan Hui, Byers Joshua, Yin Hong, Rytting Heather, Logan Suzanna, He Mai, Yu Zhongxin, Wang Dehua, Mangray Shamlal, Zhou Shengmei
Department of Pathology and Laboratory Medicine, USC/LAC+USC Medical Center Los Angeles, CA, USA.
Department of Pathology and Laboratory Medicine, Children's Hospital Los Angeles CA, USA.
Int J Clin Exp Pathol. 2023 Feb 15;16(2):32-39. eCollection 2023.
Diagnosis of angiomatoid fibrous histiocytoma (AFH) can be challenging due to its variable histologic features and a lack of highly sensitive and/or specific immunohistochemical markers. The utility of TLE1 and BCOR as immunohistochemical markers for AFH is not known.
We examined the spectrum of histologic features of 36 AFHs, and studied the expression of both TLE1 and BCOR in AFH and its mimics by immunohistochemical staining. Positive nuclear expression was scored semiquantitatively.
Both typical and unusual histologic features of AFHs were observed in this cohort. TLE1 was moderately to strongly positive in 36/36 AFHs, 4/4 synovial sarcomas, and 2/3 BCOR sarcomas; weakly positive in 4/6 inflammatory myofibroblastic tumors; negative in all dermatofibromas (n = 10), atypical fibrous histiocytomas (n = 5), myofibroma (n = 2) and juvenile xanthogranulomas (n = 5), with an overall sensitivity of 100%, and specificity of 71.4% for AFH. BCOR was moderately to strongly positive in 24/36 AFHs, 4/4 synovial sarcomas, 3/3 BCOR sarcomas, and 1/5 atypical fibrous histiocytomas; weakly positive in 10/36 AFHs; negative in the remaining tumors. The overall sensitivity and specificity of BCOR for AFH were 94.4% and 77.1%, respectively.
TLE1 is a highly sensitive immunohistochemical marker for AFH.
血管样纤维组织细胞瘤（AFH）的诊断具有挑战性，因其组织学特征多变且缺乏高敏感性和/或特异性免疫组化标志物。TLE1和BCOR作为AFH免疫组化标志物的效用尚不清楚。
我们检查了36例AFH的组织学特征谱，并通过免疫组化染色研究了TLE1和BCOR在AFH及其相似病变中的表达。对阳性核表达进行半定量评分。
在该队列中观察到了AFH的典型和不典型组织学特征。TLE1在36/36例AFH、4/4例滑膜肉瘤和2/3例BCOR肉瘤中呈中度至强阳性；在4/6例炎性肌纤维母细胞瘤中呈弱阳性；在所有皮肤纤维瘤（n = 10）、非典型纤维组织细胞瘤（n = 5）、肌纤维瘤（n = 2）和幼年性黄色肉芽肿（n = 5）中均为阴性，对AFH的总体敏感性为100%，特异性为71.4%。BCOR在24/36例AFH、4/4例滑膜肉瘤、3/3例BCOR肉瘤和1/5例非典型纤维组织细胞瘤中呈中度至强阳性；在10/36例AFH中呈弱阳性；在其余肿瘤中为阴性。BCOR对AFH的总体敏感性和特异性分别为94.4%和77.1%。
TLE1是AFH的一种高敏感性免疫组化标志物。
