Taillis David, Becissa Oussama, Pébarthé-Courrouilh Anthony, Renouf Elodie, Palos-Pinto Antonio, Richard Tristan, Cluzet Stéphanie
Bordeaux INP, INRAE, OENO, UMR 1366, ISVV, Université Bordeaux, F-33140 Villenave d'Ornon, France.
Bordeaux INP, INRAE, OENO, UMR 1366, ISVV, Bordeaux Sciences Agro, F-33170 Gradignan, France.
J Agric Food Chem. 2023 Mar 22;71(11):4488-4497. doi: 10.1021/acs.jafc.2c07843. Epub 2023 Mar 13.
Grapevine co-products, as canes, represent a source of compounds of interest to control vineyard diseases with a sustainable approach. We chose to study an extract that we produced from grapevine trunk and roots. This extract, enriched in complex stilbenes, strongly reduced mycelial growth and spore germination of , the fungal agent causing gray mold. The most active stilbenes were resveratrol, r-viniferin, and ε-viniferin. This grapevine extract also inhibited the production of Botrytis laccases. Conversely, Botrytis secretome metabolized resveratrol into δ-viniferin and pallidol (2 dimers); and ε-viniferin, a dimer, into hopeaphenol, r-viniferin, and r2-viniferin (3 tetramers). r-Viniferin and hopeaphenol (2 tetramers) were not metabolized. The biotransformed extract maintained an effective antimycelial activity. This study provides evidence that a grapevine extract enriched in oligomerized stilbenes exerts different anti-Botrytis activities, notwithstanding the ability of the fungus to metabolize some stilbenes.
葡萄藤副产品，如葡萄茎，是采用可持续方法防治葡萄园病害的潜在化合物来源。我们选择研究一种从葡萄树干和根部提取的提取物。这种富含复杂芪类化合物的提取物能显著抑制灰霉病菌的菌丝生长和孢子萌发。最具活性的芪类化合物是白藜芦醇、r - 葡萄素和ε - 葡萄素。这种葡萄藤提取物还能抑制灰葡萄孢漆酶的产生。相反，灰葡萄孢分泌组将白藜芦醇代谢为δ - 葡萄素和苍白素（2种二聚体）；而二聚体ε - 葡萄素则代谢为蛇葡萄素、r - 葡萄素和r2 - 葡萄素（3种四聚体）。r - 葡萄素和蛇葡萄素（2种四聚体）未被代谢。生物转化后的提取物仍保持有效的抗菌丝活性。这项研究表明，尽管真菌能够代谢某些芪类化合物，但富含低聚芪类化合物的葡萄藤提取物仍具有不同的抗灰葡萄孢活性。
