Agroécologie, AgroSup Dijon , CNRS, INRA, Université de Bourgogne , Franche-Comte , F-21000 Dijon , France.
CREA Research Centre for Viticulture and Enology, Via XXVIII Aprile 26 , Conegliano , Treviso 31015 , Italy.
J Agric Food Chem. 2019 May 15;67(19):5512-5520. doi: 10.1021/acs.jafc.8b07098. Epub 2019 May 2.
Crude extracts of Vitis vinifera canes represent a natural source of stilbene compounds with well characterized antifungals properties. In our trials, exogenous application of a stilbene extract (SE) obtained from grape canes on grapevine leaves reduces the necrotic lesions caused by Botrytis cinerea. The SE showed to possess a direct antifungal activity by inhibiting the mycelium growth. The activation of some grapevine defense mechanism was also investigated. HO production and activation of mitogen-activated protein kinase (MAPK) phosphorylation cascades as well as accumulation of stilbenoid phytoalexins were explored on grapevine cell suspension. Moreover, the transcription of genes encoding for proteins affecting defense responses was analyzed on grapevine plants. The SE induced some grapevine defense mechanisms including MAPK activation, and the expression of pathogenesis-related (PR) genes and of a gene encoding the glutathione-S-transferase 1 ( GST1) . By contrast, treatment of grapevine leaves with SE negatively regulates de novo stilbene production.
葡萄藤的粗提取物是具有良好抗真菌特性的芪类化合物的天然来源。在我们的试验中，将从葡萄藤中提取的芪提取物（SE）施用于葡萄叶片上，可减少由灰葡萄孢引起的坏死性损伤。SE 表现出直接的抗真菌活性，抑制菌丝生长。还研究了一些葡萄防御机制的激活。在葡萄悬浮细胞中探索了 HO 的产生和丝裂原活化蛋白激酶（MAPK）磷酸化级联的激活以及芪类植物抗毒素的积累。此外，还分析了葡萄植株上影响防御反应的蛋白质编码基因的转录。SE 诱导了一些葡萄防御机制，包括 MAPK 的激活，以及病程相关（PR）基因和谷胱甘肽-S-转移酶 1（GST1）编码基因的表达。相比之下，用 SE 处理葡萄叶片会负调控新的芪类物质的产生。
