Liu Yuhan, Chen Junwei, Zhang Ming, Guo Wei, Feng Changzeng, Liu Jiansheng, Xu Lilan, Gao Na, Ma Shaohui
Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming, People's Republic of China.
Yunnan Key Laboratory of Vaccine Research Development on Severe Infectious Disease, Kunming, People's Republic of China.
J Med Virol. 2023 Mar;95(3):e28669. doi: 10.1002/jmv.28669.
Hand, foot, and mouth disease (HFMD) is a common pediatric infectious illness caused by enteroviruses (EVs). EV-A serotypes are the main pathogens associated with HFMD. In this study, 213 stool samples from 213 children with severe HFMD in Yunnan, China in 2013, 2015, and 2016 were further analyzed retrospectively for EV-B infection. A total of 70.0% of the specimens tested positive for EV.20 EV serotypes were detected. The predominant serotype was enterovirus A71 (EV-A71, 27.7%), followed by coxsackievirus B4 (CV-B4, 16.4%), CV-A16 (9.9%), CV-B5 (6.6%), and Echovirus 9 (E-9,4.7%). EV-A and EV-B accounted for 45.1% and 41.3%, respectively. Among the positive specimens, 28.6% were CV-Bs. Co-infection was present in 19.3% of these cases. In the study, CV-B5 and the majority of CV-B4 isolates belonged to genotypes VI and C3, respectively. This result indicates that EV-B, especially CV-Bs, might be the important agents associated with HFMD and this knowledge will contribute to the prevention and treatment of the disease.
手足口病（HFMD）是一种由肠道病毒（EVs）引起的常见儿科传染病。肠道病毒A组血清型是与手足口病相关的主要病原体。在本研究中，对2013年、2015年和2016年中国云南213例重症手足口病患儿的213份粪便样本进行回顾性分析，以检测肠道病毒B组（EV-B）感染情况。共有70.0%的标本检测出肠道病毒呈阳性。检测到20种肠道病毒血清型。优势血清型为肠道病毒A71型（EV-A71，27.7%），其次是柯萨奇病毒B4型（CV-B4，16.4%）、柯萨奇病毒A16型（CV-A16，9.9%）、柯萨奇病毒B5型（CV-B5，6.6%）和埃可病毒9型（E-9，4.7%）。肠道病毒A组和肠道病毒B组分别占45.1%和41.3%。在阳性标本中，28.6%为柯萨奇病毒B组。19.3%的病例存在共感染情况。在本研究中，柯萨奇病毒B5型和大多数柯萨奇病毒B4型分离株分别属于基因型VI和C3。该结果表明，肠道病毒B组，尤其是柯萨奇病毒B组，可能是与手足口病相关的重要病原体，这一认识将有助于该病的预防和治疗。
