Department of Environmental, Biological and Pharmaceutical Science and Technology, University of Campania "Luigi Vanvitelli", 81100 Caserta, Italy.
Department of Pharmacy, University of Naples "Federico II", 80131 Naples, Italy.
Ultrason Sonochem. 2023 May;95:106360. doi: 10.1016/j.ultsonch.2023.106360. Epub 2023 Mar 8.
Herein, we developed an innovative and easily accessible solid-phase synthetic protocol for Peptide Nucleic Acid (PNA) oligomers by systematically investigating the ultrasonication effects in all steps of the PNA synthesis (US-PNAS). When compared with standard protocols, the application of the so-obtained US-PNAS approach succeeded in improving the crude product purities and the isolated yields of different PNA, including small or medium-sized oligomers (5-mer and 9-mer), complex purine-rich sequences (like a 5-mer Guanine homoligomer and the telomeric sequence TEL-13) and longer oligomers (such as the 18-mer anti-IVS2-654 PNA and the 23-mer anti-mRNA 155 PNA). Noteworthy, our ultrasound-assisted strategy is compatible with the commercially available PNA monomers and well-established coupling reagents and only requires the use of an ultrasonic bath, which is a simple equipment generally available in most synthetic laboratories.
在此，我们通过系统研究 PNA 合成（US-PNAS）的所有步骤中的超声作用，开发了一种用于肽核酸（PNA）寡聚物的创新且易于获取的固相合成方案。与标准方案相比，所获得的 US-PNAS 方法的应用成功地提高了不同 PNA 的粗产物纯度和分离产率，包括小或中寡聚物（5 聚体和 9 聚体）、复杂的富含嘌呤的序列（如 5 聚体鸟嘌呤同聚物和端粒序列 TEL-13）和更长的寡聚物（如 18 聚体抗-IVS2-654 PNA 和 23 聚体抗-mRNA155 PNA）。值得注意的是，我们的超声辅助策略与市售的 PNA 单体和成熟的偶联试剂兼容，仅需要使用超声波浴，这是大多数合成实验室通常都具备的简单设备。
