使用Ames自动分析仪定量测定毛细血管血中的苯妥英和苯巴比妥。

Quantitative determination of phenytoin and phenobarbital in capillary blood by Ames Seralyzer.

作者信息

Graves N M, Holmes G B, Leppik I E, Galligher T K, Parker D R

机构信息

University of Minnesota, College of Pharmacy, Minneapolis.

出版信息

Epilepsia. 1987 Nov-Dec;28(6):713-6. doi: 10.1111/j.1528-1157.1987.tb03705.x.

DOI:10.1111/j.1528-1157.1987.tb03705.x

PMID:3691418

Abstract

We evaluated the use of serum obtained from capillary blood specimens with the Seralyzer ARIS (AMES Division Miles Laboratories) assay for phenytoin (PHT) and phenobarbital (PB). The Ames Seralyzer method is capable of measuring PHT and PB concentrations in 10 microliter of serum. Therefore, it may be used to measure concentrations of these drugs in small blood samples, such as obtained by skin puncture and capillary tube collection. In this study, simultaneous venous and capillary samples were obtained from 90 patients with epilepsy. Seralyzer results were compared with serum values from venous blood determined with a gas chromatographic (GC) method. Comparison of PHT values obtained by Seralyzer (Ys) versus GC by linear regression gave the equation: Ys = 0.36 + 0.88 X (r2 = 0.93, n = 63). The mean difference between the Seralyzer and GC was -0.96 micrograms/ml (p less than 0.001). For PB, the equation was: Ys = -0.53 + 1.07 X (r2 = 0.93, n = 31) with a mean difference of +1.28 micrograms/ml (p = 0.02).

摘要

我们评估了使用Seralyzer ARIS（AMES分部迈尔斯实验室）检测法对从毛细血管血标本中获取的血清进行苯妥英（PHT）和苯巴比妥（PB）检测的情况。Ames Seralyzer检测法能够测定10微升血清中的PHT和PB浓度。因此，它可用于测量从小血样（如通过皮肤穿刺和毛细管采集获得的血样）中这些药物的浓度。在本研究中，从90例癫痫患者中同时采集静脉血和毛细血管血样本。将Seralyzer检测结果与用气相色谱（GC）法测定的静脉血血清值进行比较。通过线性回归比较Seralyzer（Ys）获得的PHT值与GC获得的PHT值，得到方程：Ys = 0.36 + 0.88X（r2 = 0.93，n = 63）。Seralyzer和GC之间的平均差异为 -0.96微克/毫升（p < 0.001）。对于PB，方程为：Ys = -0.53 + 1.07X（r2 = 0.93，n = 31），平均差异为 +1.28微克/毫升（p = 0.02）。