Center for Low-Temperature Plasma Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan.
Innovative Technology Laboratories, AGC Inc., 1-1 Suehirocho Tsurumi-ku, Yokohama, 230-0045, Japan.
Sci Rep. 2023 Mar 13;13(1):4130. doi: 10.1038/s41598-023-31265-9.
Various liquid biopsy methods have been developed for the non-invasive and early detection of diseases. In particular, the detection of circulating tumor cells (CTCs) and cancer-associated fibroblasts (CAFs) in blood has been receiving a great deal of attention. We have been developing systems and materials to facilitate such liquid biopsies. In this study, we further developed glass filters (with various patterns of holes, pitches, and non-adhesive coating) that can capture CTCs, but not white blood cells. We optimized the glass filters to capture CTCs, and demonstrated that they could be used to detect CTCs from lung cancer patients. We also used the optimized glass filters for detecting CAFs. Additionally, we further developed a system for visualizing the captured cells on the glass filters. Finally, we demonstrated that we could directly culture the captured cells on the glass filters. Based on these results, our high-performance glass filters appear to be useful for capturing and culturing CTCs and CAFs for further examinations.
已经开发出各种液体活检方法，用于疾病的非侵入性和早期检测。特别是，血液中循环肿瘤细胞（CTCs）和癌相关成纤维细胞（CAFs）的检测受到了广泛关注。我们一直在开发系统和材料，以促进这些液体活检。在这项研究中，我们进一步开发了玻璃滤器（具有各种孔、间距和非粘附涂层的图案），可以捕获 CTCs，但不能捕获白细胞。我们优化了玻璃滤器以捕获 CTCs，并证明它们可用于检测肺癌患者的 CTCs。我们还使用优化后的玻璃滤器检测 CAFs。此外，我们进一步开发了一种可视化玻璃滤器上捕获细胞的系统。最后，我们证明我们可以直接在玻璃滤器上培养捕获的细胞。基于这些结果，我们的高性能玻璃滤器似乎可用于捕获和培养 CTCs 和 CAFs，以进行进一步检查。
