Yancheng TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Yancheng 224000, China.
Hubei University of Chinese Medicine, Wuhan 430000, China.
J Zhejiang Univ Sci B. 2023 Mar 15;24(3):221-231. doi: 10.1631/jzus.B2200221.
Metabolic reprogramming is a common phenomenon in cancer, with aerobic glycolysis being one of its important characteristics. Hypoxia-inducible factor-1α () is thought to play an important role in aerobic glycolysis. Meanwhile, naringin is a natural flavanone glycoside derived from grapefruits and many other citrus fruits. In this work, we identified glycolytic genes related to by analyzing the colon cancer database. The analysis of extracellular acidification rate and cell function verified the regulatory effects of overexpression on glycolysis, and the proliferation and migration of colon cancer cells. Moreover, naringin was used as an inhibitor of colon cancer cells to illustrate its effect on function. The results showed that the and enolase 2 () levels in colon cancer tissues were highly correlated, and their high expression indicated a poor prognosis for colon cancer patients. Mechanistically, directly binds to the DNA promoter region and upregulates the transcription of ; ectopic expression of increased aerobic glycolysis in colon cancer cells. Most importantly, we found that the appropriate concentration of naringin inhibited the transcriptional activity of , which in turn decreased aerobic glycolysis in colon cancer cells. Generally, naringin reduces glycolysis in colon cancer cells by reducing the transcriptional activity of and the proliferation and invasion of colon cancer cells. This study helps to elucidate the relationship between colon cancer progression and glucose metabolism, and demonstrates the efficacy of naringin in the treatment of colon cancer.
代谢重编程是癌症的一种常见现象，其中有氧糖酵解是其重要特征之一。缺氧诱导因子-1α（）被认为在有氧糖酵解中发挥重要作用。同时，柚皮苷是一种天然的类黄酮糖苷，来源于葡萄柚和许多其他柑橘类水果。在这项工作中，我们通过分析结肠癌数据库鉴定了与相关的糖酵解基因。通过分析细胞外酸化率和细胞功能，验证了过表达对糖酵解、结肠癌细胞增殖和迁移的调节作用。此外，柚皮苷被用作结肠癌细胞的抑制剂，以说明其对功能的影响。结果表明，结肠癌组织中水平与烯醇酶 2（）高度相关，其高表达表明结肠癌患者预后不良。机制上，直接结合到 DNA 启动子区域，上调转录；过表达增加了结肠癌细胞中的有氧糖酵解。最重要的是，我们发现柚皮苷的适当浓度抑制了的转录活性，进而降低了结肠癌细胞中的有氧糖酵解。总的来说，柚皮苷通过降低转录活性和结肠癌细胞的增殖和侵袭来减少结肠癌细胞中的糖酵解。这项研究有助于阐明结肠癌进展与葡萄糖代谢之间的关系，并证明了柚皮苷在结肠癌治疗中的疗效。
