Luo Yong, Zhou Mengyun, Fan Chuan, Song Yongchao, Wang Lirong, Xu Tailin, Zhang Xueji
School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen, Guangdong 518060, P.R. China.
Beijing Key Laboratory for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing 100083, P.R. China.
Anal Chem. 2023 Mar 28;95(12):5316-5322. doi: 10.1021/acs.analchem.2c05381. Epub 2023 Mar 14.
Active enrichment can detect nucleic acid at ultra-low concentrations without relatively time-consuming polymerase chain reaction (PCR), which is an important development direction for future rapid nucleic acid detection. Here, we reported an integrated active enrichment platform for direct hand-held detection of nucleic acid of COVID-19 in nanoliter samples without PCR. The platform consists of a capillary-assisted liquid-carrying system for sampling, integrated circuit system for ultrasound output, and cell-phone-based surface-enhanced Raman scattering (SERS) system. Considering the acoustic responsiveness and SERS-enhanced performance, gold nanorods were selected for biomedical applications. Functionalized gold nanorods can effectively capture and enrich biomarkers under ultrasonic aggregation. Such approaches can actively assemble gold nanorods in 1-2 s and achieved highly sensitive (6.15 × 10 M) SERS detection of COVID-19 biomarkers in nanoliter (10 L) samples within 5 min. We further demonstrated the high stability, repeatability, and selectivity of the platform, and validated its potential for the detection of throat swab samples. This simple, portable, and ultra-trace integrated active enrichment detection platform is a promising diagnostic tool for the direct and rapid detection of COVID-19.
主动富集能够在无需耗时的聚合酶链反应（PCR）的情况下检测超低浓度的核酸，这是未来快速核酸检测的一个重要发展方向。在此，我们报道了一种集成主动富集平台，可在无PCR的情况下直接对纳升样品中的新冠病毒核酸进行手持式检测。该平台由用于采样的毛细管辅助液体输送系统、用于超声输出的集成电路系统以及基于手机的表面增强拉曼散射（SERS）系统组成。考虑到声学响应性和SERS增强性能，选择了金纳米棒用于生物医学应用。功能化的金纳米棒能够在超声聚集作用下有效捕获和富集生物标志物。此类方法能够在1至2秒内主动组装金纳米棒，并在5分钟内实现对纳升（10微升）样品中的新冠病毒生物标志物的高灵敏度（6.15×10⁻⁹ M）SERS检测。我们进一步证明了该平台的高稳定性、可重复性和选择性，并验证了其在检测咽拭子样品方面的潜力。这个简单、便携且超痕量的集成主动富集检测平台是用于直接快速检测新冠病毒的一种很有前景的诊断工具。
