Antonenkov V D, Pirozhkov S V, Panchenko L F
All-Union Research Center for Medico-Biological Problems of Narcology, Moscow, USSR.
FEBS Lett. 1987 Nov 30;224(2):357-60. doi: 10.1016/0014-5793(87)80484-2.
To elucidate a possible role of membrane-bound aldehyde dehydrogenase in the detoxication of aldehydic products of lipid peroxidation, the substrate specificity of the highly purified microsomal enzyme was investigated. The aldehyde dehydrogenase was active with different aliphatic aldehydes including 4-hydroxyalkenals, but did not react with malonic dialdehyde. When Fe/ADP-ascorbate-induced lipid peroxidation of arachidonic acid was carried out in an in vitro system, the formation of products which react with microsomal aldehyde dehydrogenase was observed parallel with malonic dialdehyde accumulation.
为阐明膜结合醛脱氢酶在脂质过氧化醛产物解毒过程中可能的作用，对高度纯化的微粒体酶的底物特异性进行了研究。该醛脱氢酶对包括4-羟基烯醛在内的不同脂肪醛具有活性，但不与丙二醛反应。当在体外系统中进行铁/ADP-抗坏血酸诱导的花生四烯酸脂质过氧化时，观察到与微粒体醛脱氢酶反应的产物形成与丙二醛积累同时发生。
