Lindahl R, Petersen D R
Department of Biochemistry and Molecular Biology, University of South Dakota, School of Medicine, Vermillion 57069.
Biochem Pharmacol. 1991 Jun 1;41(11):1583-7. doi: 10.1016/0006-2952(91)90157-z.
A large number of different unsaturated, saturated and hydroxylated aliphatic aldehydes can be generated during the peroxidation of cellular lipids. This study examined the kinetic properties of purified Class 3 rat aldehyde dehydrogenase (ALDH) with respect to the oxidation of various lipid aldehyde substrates. It also compared the substrate preference of the prototypic Class 3 ALDH with that of the constitutive rat microsomal aldehyde dehydrogenase. The results suggest that (1) microsomal ALDH is a member of the Class 3 aldehyde dehydrogenase family, and (2) the physiological role of the Class 3 ALDHs, including the microsomal form, is the oxidation of medium (6 to 9 carbon) chain length saturated and unsaturated aldehydes generated by the peroxidation of cellular lipids. Short chain aliphatic aldehydes, such as a malondialdehyde and 4-hydroxyalkenals, are not substrates for the Class 3 aldehyde dehydrogenases.
细胞脂质过氧化过程中可产生大量不同的不饱和、饱和及羟基化脂肪醛。本研究检测了纯化的3类大鼠醛脱氢酶（ALDH）对各种脂质醛底物氧化的动力学特性。还比较了原型3类ALDH与组成型大鼠微粒体醛脱氢酶的底物偏好性。结果表明：（1）微粒体ALDH是3类醛脱氢酶家族的成员；（2）包括微粒体形式在内的3类ALDH的生理作用是氧化细胞脂质过氧化产生的中等（6至9个碳）链长的饱和及不饱和醛。短链脂肪醛，如丙二醛和4-羟基烯醛，不是3类醛脱氢酶的底物。
