Ohshima M, Matsuoka M, Yamamoto N, Tanaka Y, Kano-Murakami Y, Ozeki Y, Kato A, Harada N, Ohashi Y
National Institute of Agrobiological Resources, Tsukuba Science City, Ibaraki, Japan.
FEBS Lett. 1987 Dec 10;225(1-2):243-6. doi: 10.1016/0014-5793(87)81166-3.
A gene encoding one of the pathogenesis-related proteins, PR1a, and two related pseudogenes were isolated from Nicotiana tabacum. The cloned PR1a gene (pPR-gamma) and one of the pseudogenes (pPR-alpha) were sequenced and found to have similar structures. The sequence of pPR-gamma was quite similar to that of the cDNA clone of PR1a. The plasmid pPR-gamma did not contain an intron and had a typical promoter sequence in the 5'-flanking region.
从烟草中分离出一个编码病程相关蛋白PR1a的基因以及两个相关的假基因。对克隆的PR1a基因(pPR-γ)和其中一个假基因(pPR-α)进行了测序,发现它们具有相似的结构。pPR-γ的序列与PR1a的cDNA克隆序列非常相似。质粒pPR-γ不包含内含子,并且在5'侧翼区域具有典型的启动子序列。