Department of Chemical Engineering & Technology, Indian Institute of Technology (BHU) Varanasi, Varanasi 221005, Uttar Pradesh, India.
Department of Environmental Science, Jamia Millia Islamia, (A Central University), New Delhi 110025, India.
Int J Biol Macromol. 2023 May 15;237:124033. doi: 10.1016/j.ijbiomac.2023.124033. Epub 2023 Mar 12.
Cellulases are among the most in-demand bioprocess enzymes, and the high cost of production, combined with their low enzymatic activity, is the main constraint, particularly in the biofuels industry. As a result, low-cost enzyme production modes with high activity and stability have emerged as the primary focus of research. Here, a method for producing a graphene like carbon nanostructure (GLCNs) has been investigated utilizing paddy straw (Ps), and its physicochemical characteristics have been examined using a variety of techniques including XRD, FT-IR, SEM and TEM. Further, the pretreatment of Ps feedstock for cellulase production was done using diluted waste KOH liquid collected during the preparation of the GLCNs. To increase the production and stability of the enzyme, newly prepared GLCNs is utilized as a nanocatalyst. Using 15 mg of GLCNs, 35 IU/gds FP activity was seen after 72 h, followed by 158 IU/gds EG and 114 IU/gds BGL activity in 96 h. This nanocatalyst supported enzyme was thermally stable at 70 °C up to 15 h and exhibited stability at pH 7.0 for 10 h by holding 66 % of its half-life.
纤维素酶是需求量最大的生物加工酶之一，其生产成本高，酶活性低，这是主要的限制因素，特别是在生物燃料行业。因此，具有高活性和稳定性的低成本酶生产模式已成为研究的重点。在这里，研究了一种利用稻秆（Ps）生产类石墨烯碳纳米结构（GLCNs）的方法，并使用多种技术（包括 XRD、FT-IR、SEM 和 TEM）对其物理化学特性进行了研究。此外，还利用在 GLCNs 制备过程中收集的稀释废 KOH 液体对 Ps 原料进行了纤维素酶生产的预处理。为了提高酶的产量和稳定性，新制备的 GLCNs 被用作纳米催化剂。使用 15mg 的 GLCNs，在 72 小时后观察到 35IU/gds FP 活性，随后在 96 小时内观察到 158IU/gds EG 和 114IU/gds BGL 活性。这种纳米催化剂支持的酶在 70°C 下稳定 15 小时，在 pH7.0 下稳定 10 小时，保持了其半衰期的 66%。
