[血管内皮生长因子（VEGF）和血管内皮生长因子受体1（VEGFR1）通路相关基因多态性与子痫前期风险的关联]
[Association of polymorphisms of VEGF and VEGFR1 pathways related genes and risk of pre-eclampsia].
作者信息
Li Li, Guo Xiangcui, Chen Beibei, Gao Zhihui, Liu Juan, Wang Qiangqing
机构信息
Department of Gynecology and Oncology, Xinxiang Central Hospital, Xinxiang, Henan 453000, China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2022 Aug 10;39(8):893-897. doi: 10.3760/cma.j.cn511374-20210623-00534.
OBJECTIVE
To assess the association of single nucleotide polymorphisms (SNPs) of vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor 1 (VEGFR1) pathways-related genes and the risk of pre-eclampsia.
METHODS
In total 178 pregnant women with pre-eclampsia (case group) and 100 healthy pregnant women (control group) during the third trimester were enrolled. The SNPs of VEGF rs3025039, rs2010963 and VEGFR1 rs3812867, rs55875014 and rs722503 loci were determined by PCR-restriction fragment length polymorphism (PCR-RFLP) assay. The levels of serum VEGF and sVEGFR1 were also determined. And their association with pre-eclampsia was analyzed.
RESULTS
The systolic blood pressure, diastolic blood pressure and sVEGFR1 of the case group were significantly higher than those of the control group, while the VEGF level was significantly lower than that in the control group (P<0.05). Allelic frequencies of the VEGF (rs3025039, rs2010963) and VEGFR1 (rs3812867, rs55875014, rs722503) have fit the Hardy-Weinberg equilibrium (P>0.05). The frequency of T allele of VEGF at rs3025039 locus in the case group was higher than that in the control group (P<0.05). There were significant differences in VEGF at rs3025039 locus under dominant and co-dominant models in case group (P<0.05). Compared with those with CC, the risk was higher in patients with CT or TT genotypes (P<0.05). The systolic and diastolic blood pressure and sVEGFR1 in pre-eclampsia pregnant women with CT or TT genotypes were significantly higher than those with the CC genotype, while their VEGF level was significantly lower (P<0.05). No significant difference was found in allelic frequencies of other four loci between the two groups (P>0.05).
CONCLUSION
Polymorphisms of rs3025039 locus of VEGF gene is associated with the occurrence of pre-eclampsia. The variant at this locus may affect the activity of VEGF and influence the development of pre-eclampsia.
目的
评估血管内皮生长因子（VEGF）和血管内皮生长因子受体1（VEGFR1）通路相关基因的单核苷酸多态性（SNP）与子痫前期风险的关联。
方法
纳入178例孕晚期子痫前期孕妇（病例组）和100例健康孕妇（对照组）。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析法检测VEGF rs3025039、rs2010963以及VEGFR1 rs3812867、rs55875014和rs722503位点的SNP。同时测定血清VEGF和可溶性VEGFR1（sVEGFR1）水平，并分析它们与子痫前期的关联。
结果
病例组的收缩压、舒张压和sVEGFR1显著高于对照组，而VEGF水平显著低于对照组（P<0.05）。VEGF（rs3025039、rs2010963）和VEGFR1（rs3812867、rs55875014、rs722503）的等位基因频率符合Hardy-Weinberg平衡（P>0.05）。病例组中VEGF rs3025039位点的T等位基因频率高于对照组（P<0.05）。病例组中rs3025039位点在显性和共显性模型下的VEGF存在显著差异（P<0.05）。与CC基因型患者相比，CT或TT基因型患者的风险更高（P<0.05）。CT或TT基因型的子痫前期孕妇的收缩压、舒张压和sVEGFR1显著高于CC基因型孕妇，而其VEGF水平显著更低（P<0.05）。两组间其他四个位点的等位基因频率无显著差异（P>0.05）。
结论
VEGF基因rs3025039位点的多态性与子痫前期的发生有关。该位点的变异可能影响VEGF的活性并影响子痫前期的发展。
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