Department of Gynaecology, Linyi Cancer Hospital, Linyi, People's Republic of China.
Department of Galactophore, Linyi Cancer Hospital, Linyi, People's Republic of China.
J Hum Genet. 2017 Dec;62(12):1065-1071. doi: 10.1038/jhg.2017.85. Epub 2017 Sep 7.
We investigated the relationship between gonadotropin-releasing hormone receptor (GnRHR) gene polymorphisms and outcome of patients with polycystic ovary syndrome (PCOS) undergoing in vitro fertilization and embryo transfer (IVF-ET). PCOS patients undergoing IVF-ET were selected, and infertile patients due to dysfunctional oviducts served as controls. GnRHR gene polymorphisms were detected using the polymerase chain reaction-restriction fragment length polymorphism assay. Gene-gene interaction and linkage disequilibrium tests were performed using the SHEsis software. Logistic regression analysis was performed to evaluate factors affecting outcome of patients undergoing IVF-ET. The PCOS group showed more patients with CC+CT genotypes rs12644822, rs3756159 and rs13138607 than the control group, and CC+CT genotypes and C alleles from three positions enhanced risk of PCOS. Patients with CC+CT genotypes from three positions exhibited increased serum luteinizing hormone (LH), LH/follicle-stimulating hormone (FSH), testosterone (T) and follicles than those with TT genotypes. The haplotype analysis indicated that CCC, CCT and TCC haplotypes increased the risk of PCOS, while TCT, TTC and TTT haplotypes lowered the risk. After IVF-ET treatment, patients with CC+CT genotypes of three positions in the GnRHR gene had a lower pregnancy rate than patients with TT genotypes. Logistic regression analysis indicated that CC+CT genotypes rs12644822, rs3756159 and rs13138607 were risk factor for patients undergoing IVF-ET. In conclusion, these findings demonstrate that CC+CT genotypes rs12644822C>T, rs3756159C>T and rs13138607C>T in the GnRHR gene may contribute to a decreased pregnancy rate for PCOS patients after IVF-ET.
我们研究了促性腺激素释放激素受体（GnRHR）基因多态性与行体外受精和胚胎移植（IVF-ET）的多囊卵巢综合征（PCOS）患者结局的关系。选择行 IVF-ET 的 PCOS 患者，以因输卵管功能障碍而不孕的患者作为对照。采用聚合酶链反应-限制性片段长度多态性分析检测 GnRHR 基因多态性。采用 SHEsis 软件进行基因-基因相互作用和连锁不平衡检验。采用 logistic 回归分析评估影响 IVF-ET 患者结局的因素。PCOS 组 rs12644822、rs3756159 和 rs13138607 的 CC+CT 基因型患者多于对照组，三个位置的 CC+CT 基因型和 C 等位基因增加了 PCOS 的发病风险。三个位置 CC+CT 基因型患者的血清黄体生成素（LH）、LH/卵泡刺激素（FSH）、睾酮（T）和卵泡数高于 TT 基因型患者。单体型分析表明，CCC、CCT 和 TCC 单体型增加了 PCOS 的发病风险，而 TCT、TTC 和 TTT 单体型降低了发病风险。行 IVF-ET 治疗后，GnRHR 基因三个位置 CC+CT 基因型患者的妊娠率低于 TT 基因型患者。logistic 回归分析表明，rs12644822、rs3756159 和 rs13138607 的 CC+CT 基因型是 IVF-ET 患者的危险因素。总之，这些发现表明 GnRHR 基因 rs12644822C>T、rs3756159C>T 和 rs13138607C>T 的 CC+CT 基因型可能导致 PCOS 患者 IVF-ET 后妊娠率降低。
