Department of Restorative Dentistry, College of Dentistry, University of Illinois Chicago, Chicago, Illinois, USA.
Department of Pediatric Dentistry, College of Dentistry, University of Illinois Chicago, Chicago, Illinois, USA.
J Biomed Mater Res B Appl Biomater. 2023 Aug;111(8):1480-1487. doi: 10.1002/jbm.b.35247. Epub 2023 Mar 15.
To improve surface properties of poly(methyl methacrylate) (PMMA) using nano-ceramic coatings and assess microbial adherence after long-term use of a chemical cleanser. Thirty-six PMMA samples were fabricated, polished and coated with a nano-thin TiO or mixed TiO /ZrO , with uncoated samples as controls. Six samples in each group (n = 12) were soaked in Polident denture cleaner 180 times for 30 min, while six were soaked in deionized water. Surface roughness of PMMA before and after being soaked in Polident was assessed. All samples were subsequently exposed to Candida albicans for 6 h and the adherent cells were determined by viable colony count. Two-way analysis of variance was performed for statistical analysis. No significant difference in surface roughness was noted between the uncoated and coated PMMA before soaking. After soaking, surface roughness of the uncoated PMMA increased from 0.164 to 0.532 μm (p < .05). No significant change was observed for TiO -coated (0.105-0.143 μm) or TiO /ZrO -coated PMMA (0.104-0.141 μm). Attachment of C. albicans to PMMA soaked in water showed significantly less attachment to both TiO -coated (1.4 × 10 cfu/ml) and TiO /ZrO -coated PMMA (1.6 × 10 cfu/ml) than to the uncoated PMMA (2.6 × 10 cfu/ml). After soaking in Polident, the uncoated PMMA had significantly less C. albicans attachment than coated samples. Less attachment was noted on the TiO /ZrO -coated PMMA then the TiO -coated samples (p < .05). Nano-ceramic TiO2 /ZrO coating of PMMA denture base material alters surface properties thus reduces oral microbial adhesion. It represents a promising alternative to the chemical disinfection for PMMA denture materials.
为改善聚甲基丙烯酸甲酯（PMMA）的表面性能，采用纳米陶瓷涂层，并评估长期使用化学清洁剂后的微生物黏附情况。制作了 36 个 PMMA 样本，对其进行抛光和涂覆纳米 TiO 或混合 TiO /ZrO 涂层，未涂层样本作为对照。每组 6 个样本（n=12）浸泡在 Polident 义齿清洁剂中 180 次，每次 30 分钟，同时浸泡在去离子水中的 6 个样本作为对照。评估 PMMA 在浸泡 Polident 前后的表面粗糙度。所有样本随后暴露于白色念珠菌 6 小时，通过活菌计数确定黏附细胞。采用双因素方差分析进行统计分析。浸泡前，未涂层和涂层 PMMA 的表面粗糙度无显著差异。浸泡后，未涂层 PMMA 的表面粗糙度从 0.164 增加到 0.532μm（p<0.05）。TiO 涂层（0.105-0.143μm）或 TiO /ZrO 涂层 PMMA（0.104-0.141μm）的表面粗糙度无显著变化。浸泡在水中的 PMMA 黏附白色念珠菌的情况表明，TiO 涂层（1.4×10 cfu/ml）和 TiO /ZrO 涂层 PMMA（1.6×10 cfu/ml）的黏附明显少于未涂层 PMMA（2.6×10 cfu/ml）。浸泡在 Polident 后，未涂层 PMMA 的白色念珠菌黏附明显少于涂层样本。TiO /ZrO 涂层 PMMA 的黏附明显少于 TiO 涂层样本（p<0.05）。PMMA 义齿基托材料的纳米陶瓷 TiO2 /ZrO 涂层改变表面性能，从而减少口腔微生物黏附。它为 PMMA 义齿材料的化学消毒提供了一种有前途的替代方法。
