Efeoglu Ozseker Pinar, Yucel Sevinc Puren, Daglioglu Nebile
Department of Forensic Medicine, Faculty of Medicine, Cukurova University, Adana, Turkey.
Department of Biostatistics, Faculty of Medicine, Cukurova University, Adana, Turkey.
J Immunoassay Immunochem. 2023 Jul 4;44(4):313-325. doi: 10.1080/15321819.2023.2189451. Epub 2023 Mar 15.
Immunoassay tests are used in clinical and forensic toxicology laboratories to determine illicit drug use in biological samples. Therefore, this study aims to optimize the cutoff concentrations of DOA I Plus in the blood and compare the LC-MS/MS results. 680 authentic forensic whole blood specimens with Randox Evidence DOA I Plus array were screened for drug of abuse and confirmed by LC-MS/MS. Regarding the manufacturer recommended threshold values, 139 out of 680 authentic blood samples were positive for one or more analytes, while 541 were negative. Nearly all of the 139 positive blood samples confirmed by LC-MS/MS were true positive for one or more analytes, while 522 of the 541 negative blood samples were true negative. The overall sensitivity and the specificity were 87.8% and 99.6%, respectively. THC was considered in detail, and a receiver operator characteristic curve analysis was performed to determine the optimum cutoff for THC, as it accounts for 78% of all positive results according to the manufacturer's recommended thresholds. The optimal threshold value for THC was determined at a concentration of 23 ng/mL, while these values for other parameters were defined as recommended by the manufacturer.
免疫分析测试用于临床和法医毒理学实验室，以确定生物样本中是否存在非法药物使用情况。因此，本研究旨在优化DOA I Plus在血液中的临界浓度，并比较液相色谱-串联质谱（LC-MS/MS）的结果。对680份使用朗道（Randox）Evidence DOA I Plus检测组合的真实法医全血样本进行了滥用药物筛查，并通过LC-MS/MS进行确认。根据制造商推荐的阈值，680份真实血样中有139份一种或多种分析物呈阳性，541份呈阴性。经LC-MS/MS确认的139份阳性血样中，几乎所有样本的一种或多种分析物均为真阳性，而541份阴性血样中有522份为真阴性。总体灵敏度和特异性分别为87.8%和99.6%。对四氢大麻酚（THC）进行了详细分析，并进行了受试者工作特征曲线分析，以确定THC的最佳临界值，因为根据制造商推荐的阈值，THC占所有阳性结果的78%。THC的最佳阈值确定为浓度23纳克/毫升，而其他参数的值则按照制造商的建议进行定义。
