Akbaba Murat, Isir Aysun Baransel
Department of Forensic Medicine, Faculty of Medicine, Gaziantep University, 27310 Gaziantep, Turkey.
Diagnostics (Basel). 2025 Jan 23;15(3):269. doi: 10.3390/diagnostics15030269.
: This study aimed to evaluate the efficacy and reliability of LC-MS/MS and biochip-based screening in detecting narcotics from blood and urine samples. : In this single-center study, a total of 250 subjects provided urine and/or blood samples: 234 provided urine and blood samples, and 16 provided only blood samples. Biochip-based narcotics screening was performed on 234 urine and 16 blood samples, and all samples were analyzed using tandem liquid chromatography-mass spectrometry (LC-MS/MS). : The two most prevalent narcotics were methamphetamine and amphetamine, with both biochip-based array and LC-MS/MS. Cohen's kappa correlation indicated a substantial agreement between LC-MS/MS and biochip-based screening for both methamphetamine (κ = 0.661, <0.001) and amphetamine (κ = 0.663, < 0.001). LC-MS/MS demonstrated near-perfect agreement between urine and blood samples for both methamphetamine (κ = 0.855, <0.001) and amphetamine (κ = 0.881, < 0.001). : Biochip-based arrays are a valid method offering a faster, less material-intensive alternative to LC-MS/MS for substance detection in forensic examination.
本研究旨在评估液相色谱-串联质谱法（LC-MS/MS）和生物芯片筛查在检测血液和尿液样本中麻醉药品方面的有效性和可靠性。在这项单中心研究中，共有250名受试者提供了尿液和/或血液样本：234人提供了尿液和血液样本，16人仅提供了血液样本。对234份尿液和16份血液样本进行了基于生物芯片的麻醉药品筛查，并使用串联液相色谱-质谱法（LC-MS/MS）对所有样本进行了分析。基于生物芯片阵列和LC-MS/MS检测出的两种最常见的麻醉药品是甲基苯丙胺和苯丙胺。科恩kappa相关性表明，LC-MS/MS与基于生物芯片的筛查在甲基苯丙胺（κ = 0.661，<0.001）和苯丙胺（κ = 0.663，<0.001）检测上具有高度一致性。LC-MS/MS在甲基苯丙胺（κ = 0.855，<0.001）和苯丙胺（κ = 0.881，<0.001）的尿液和血液样本检测上显示出近乎完美的一致性。基于生物芯片的阵列是一种有效的方法，在法医检验中，它为物质检测提供了一种比LC-MS/MS更快、材料消耗更少的替代方法。
