Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Key Clinical Laboratory of Henan Province, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Int J Lab Hematol. 2023 Aug;45(4):522-527. doi: 10.1111/ijlh.14057. Epub 2023 Mar 15.
The assessment of neutrophil dysplasia features in peripheral blood is very helpful for the early screening and diagnosis of myelodysplastic neoplasms (MDS). Cell population data (CPD) parameters generated by automated hematology analyzers can reflect morphological characteristics of blood cells. This study aimed to investigate the clinical significance of CPD parameters neutrophil (Neu) X, NeuY and NeuZ in assessing neutrophil dysplasia.
218 MDS patients were divided into two subgroups according to neutrophil morphology. The differences of neutrophil research parameters between the two MDS subgroups and the control group, consisting of 210 healthy individuals, were compared, the correlation among neutrophil research parameters and the relationship between these parameters and cell morphology in MDS patients were analyzed, and receiver operating characteristic analysis were performed.
The median values of neutrophil research parameters NeuX and NeuZ in MDS with granulocyte dysplasia group were significantly lower than those in MDS without granulocyte dysplasia group and control group (p < 0.001), and they were positively correlated (r = 0.878, p < 0.001). The area under the receiver operating characteristic curve of NeuX and NeuZ was 0.720 (95% CI: 0.643-0.796, p < 0.001) and 0.738 (95% CI: 0.665-0.811, p < 0.001), respectively. In addition, with the decrease of NeuX value, neutrophils gradually show decreased nuclear segment and/or cytoplasmic granules.
Combining NeuX and NeuZ can predict neutrophil dysplasia features of MDS in peripheral blood, and this can be an easier method to screen for the neutrophil dysplasia cases, as compared with the microscopic examination of peripheral blood and/or bone marrow smears.
外周血中性粒细胞形态异常的评估对骨髓增生异常综合征（MDS）的早期筛查和诊断非常有帮助。自动化血液分析仪生成的细胞群体数据（CPD）参数可以反映血细胞的形态特征。本研究旨在探讨CPD 参数中性粒细胞（Neu）X、NeuY 和 NeuZ 对评估中性粒细胞形态异常的临床意义。
根据中性粒细胞形态将 218 例 MDS 患者分为两组。比较两组 MDS 亚组与 210 例健康对照者之间中性粒细胞研究参数的差异，分析中性粒细胞研究参数之间的相关性以及这些参数与 MDS 患者细胞形态之间的关系，并进行受试者工作特征（ROC）分析。
粒细胞形态异常 MDS 组的中性粒细胞研究参数 NeuX 和 NeuZ 的中位数明显低于粒细胞形态正常 MDS 组和对照组（p<0.001），且二者呈正相关（r=0.878，p<0.001）。NeuX 和 NeuZ 的 ROC 曲线下面积分别为 0.720（95%CI：0.643-0.796，p<0.001）和 0.738（95%CI：0.665-0.811，p<0.001）。此外，随着 NeuX 值的降低，中性粒细胞逐渐表现出核分叶减少和/或细胞质颗粒减少。
联合 NeuX 和 NeuZ 可预测外周血 MDS 的中性粒细胞形态异常特征，与外周血和/或骨髓涂片的显微镜检查相比，这是一种更简单的筛选中性粒细胞形态异常的方法。
