Schulze A, Geiler G
Z Rheumatol. 1979 Jan-Feb;38(1-2):50-8.
Today the immuno-fluorescence technique is the simplest method to detect rheumatoid factors of all immuno-globulin classes. When applying sheep erythrocytes, standard sera may be used without exception. The airdried smear of the cells is to be incubated with rabbit anti-human-erythrocyte serum. The rheumatoid factors of the subsequently applied inactivated patients' sera localise above the amboceptor on the erythrocyte membrane. Only they bind FITC marked anti-human-gamma-globulin and cause a specific fluorescence. As a proof heteroagglutinines and crossreacting antibodies of the marked sera are first absorbed and corresponding controls are carried out. According to assessments of 101 sera approximately 20% of healthy people, approximately 50% of "definitely seronegative" patients with rheumatoid arthritis according to the agglutination method as well as 30% of other arthropathies had rheumatoid factors by these methods. A negative immunofluorescence optical test does not absolutely exclude the presence of rheumatoid factors in the serum. It is suggested that their occurrence are due to secondary reactions to the formation of immunogen antigen-antibody complexes.
如今，免疫荧光技术是检测所有免疫球蛋白类别的类风湿因子的最简单方法。使用绵羊红细胞时，标准血清可毫无例外地使用。将细胞的空气干燥涂片与兔抗人红细胞血清一起孵育。随后应用的灭活患者血清中的类风湿因子定位于红细胞膜上的介体上方。只有它们结合异硫氰酸荧光素标记的抗人γ球蛋白并产生特异性荧光。作为证据，首先吸收标记血清的异嗜性凝集素和交叉反应抗体，并进行相应的对照。根据对101份血清的评估，通过这些方法，大约20%的健康人、大约50%根据凝集法“肯定血清阴性”的类风湿性关节炎患者以及30%的其他关节病患者有类风湿因子。免疫荧光光学检测呈阴性并不能绝对排除血清中存在类风湿因子。有人认为它们的出现是由于对免疫原抗原-抗体复合物形成的继发反应。
