高压灭菌处理无法完全灭活弥漫性路易体病α-突触核蛋白的种子活性。
Autoclave treatment fails to completely inactivate DLB alpha-synuclein seeding activity.
作者信息
Han Jung-Youn, Park Kyung-Je, Park Hoo-Chang, Lee Yu-Ran, Moore Roger A, Sohn Hyun-Joo, Choi Young Pyo
机构信息
Laboratory Animal Center, Division of Research Strategy, Korea Brain Research Institute, Daegu, Republic of Korea.
Reference Laboratory for CWD, Foreign Animal Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea.
出版信息
Biochem Biophys Rep. 2023 Mar 3;34:101446. doi: 10.1016/j.bbrep.2023.101446. eCollection 2023 Jul.
Synucleinopathies are characterized by the deposition of alpha-synuclein (α-syn) aggregates in brain tissue. Pathological α-syn aggregates propagate in a prion-like manner and display prion-like biochemical properties. Using RT-QuIC, we measured α-syn seeding activity from brains of Dementia with Lewy body (DLB) patients post autoclave. Here, we show that autoclaving at 121 °C removes one to two log of α-syn seeding activity but the remaining 50% seeding dose (SD) is more than 107/mg tissue. DLB brain samples autoclaved at 132 °C still revealed an SD of approximately 106/mg tissue. Our data suggest that DLB α-syn seeds are incompletely inactivated by standard autoclave, thus highlighting the need for evaluating laboratory procedures that fully inactivate them.
突触核蛋白病的特征是α-突触核蛋白（α-syn）聚集体在脑组织中沉积。病理性α-syn聚集体以朊病毒样方式传播并表现出朊病毒样生化特性。我们使用实时无细胞感染性检测（RT-QuIC）方法，测量了路易体痴呆（DLB）患者脑标本在高压灭菌后的α-syn种子活性。在此，我们发现，121°C高压灭菌可去除一到两个对数的α-syn种子活性，但剩余的50%种子剂量（SD）仍超过10^7/毫克组织。在132°C下高压灭菌的DLB脑样本仍显示约10^6/毫克组织的SD。我们的数据表明，标准高压灭菌不能完全灭活DLB的α-syn种子，因此突出了评估能使其完全失活的实验室程序的必要性。
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