Zhu Yuliang, Liu Yucan, Chang Huanhuan, Yang Hao, Zhang Wei, Zhang Yanxiang, Sun Hongwei
School of Environmental and Material Engineering, Yantai University, Yantai, China.
School of Civil Engineering, Yantai University, Yantai, Shandong, China.
Front Bioeng Biotechnol. 2023 Feb 27;11:1107633. doi: 10.3389/fbioe.2023.1107633. eCollection 2023.
The proper operation of wastewater treatment plants is a key factor in maintaining a stable river and lake environment. Low purification efficiency in winter is a common problem in high-altitude wastewater treatment plants (WWTPs), and analysis of the microbial community involved in the sewage treatment process at high-altitude can provide valuable references for improving this problem. In this study, the bacterial communities of high- and low-altitude WWTPs were investigated using Illumina high-throughput sequencing (HTS). The interaction between microbial community and environmental variables were explored by co-occurrence correlation network. At genus level, (5.2%), (3.0%), (2.5%), and (2.5%) were the dominant genera in high-altitude group. The abundance of nitrogen and phosphorus removal bacteria were higher in high-altitude group (10.2% and 1.3%, respectively) than in low-altitude group (5.4% and 0.6%, respectively). Redundancy analysis (RDA) and co-occurrence network analysis showed that altitude, ultraviolet index (UVI), pH, dissolved oxygen (DO) and total nitrogen (TN) were the dominated environmental factors ( < 0.05) affecting microbial community assembly, and these five variables explained 21.4%, 20.3%, 16.9%, 11.5%, and 8.2% of the bacterial assembly of AS communities. The community diversity of high-altitude group was lower than that of low-altitude group, and WWTPs of high-altitude aeras had a unique microbial community structure. Low temperature and strong UVI are pivotal factors contributing to the reduced diversity of activated sludge microbial communities at high-altitudes.
污水处理厂的正常运行是维持稳定的河湖环境的关键因素。冬季净化效率低是高海拔污水处理厂普遍存在的问题，分析高海拔污水处理过程中涉及的微生物群落可为解决这一问题提供有价值的参考。在本研究中，采用Illumina高通量测序（HTS）技术对高海拔和低海拔污水处理厂的细菌群落进行了调查。通过共现相关网络探讨了微生物群落与环境变量之间的相互作用。在属水平上，（5.2%）、（3.0%）、（2.5%）和（2.5%）是高海拔组的优势属。高海拔组中氮磷去除菌的丰度（分别为10.2%和1.3%）高于低海拔组（分别为5.4%和0.6%）。冗余分析（RDA）和共现网络分析表明，海拔、紫外线指数（UVI）、pH值、溶解氧（DO）和总氮（TN）是影响微生物群落组装的主要环境因素（<0.05），这五个变量分别解释了AS群落细菌组装的21.4%、20.3%、16.9%、11.5%和8.2%。高海拔组的群落多样性低于低海拔组，高海拔地区的污水处理厂具有独特的微生物群落结构。低温和强UVI是导致高海拔活性污泥微生物群落多样性降低的关键因素。
